Characterization of Promoters Integrated in the Genome of Bovine Herpesvirus-1 (BHV-1)

Takayuki Murata, Xuenan Xuan, Haruki Otsuka

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Bovine herpesvirus-1 (BHV-1) has been used as a vector of live recombinant vaccines for cattle which express the genes of other pathogens. Because of the importance of the choice of the promoter which allows the efficient expression of the foreign genes in the BHV-1 vector, we compared the relative efficacy of various promoters integrated in the BHV-1 genome. The promoter sequences of the BHV-1 thymidine kinase (tk), gB, gC, SV40 early, and pseudorabies virus (PRV) immediate early (IE) genes were placed at the upstream of the open reading frame of the chloramphenycol acetyl transferase (CAT) gene and the promoter-CAT sequences were integrated into the tk gene of BHV-1 by homologous recombination. The promoter activity was assayed by measuring the CAT activity in the extracts of Madin Darby bovine kidney (MDBK) cells infected with the recombinant BHV-1. The PRV IE promoter was activated earlier and maintained at a higher level activity than the BHV-1 gB or gC promoters throughout the most of the growth phase of BHV-1. At the late phase, however, the activities of the BHV-1 gB and gC promoters reached the higher level. The BHV-1 tk promoter activity was low and the SV40 early promoter was hardly activated when integrated into the BHV-1 genome.

Original languageEnglish
Pages (from-to)453-457
Number of pages5
JournalJournal of Veterinary Medical Science
Volume61
Issue number5
Publication statusPublished - 01-05-1999

Fingerprint

Bovine Herpesvirus 1
Bovine herpesvirus 1
promoter regions
Genome
genome
thymidine kinase
Thymidine Kinase
Transferases
transferases
Suid Herpesvirus 1
Suid herpesvirus 1
genes
Genes
Synthetic Vaccines
Immediate-Early Genes
Simian virus 40
Homologous Recombination
homologous recombination
cattle
live vaccines

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

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abstract = "Bovine herpesvirus-1 (BHV-1) has been used as a vector of live recombinant vaccines for cattle which express the genes of other pathogens. Because of the importance of the choice of the promoter which allows the efficient expression of the foreign genes in the BHV-1 vector, we compared the relative efficacy of various promoters integrated in the BHV-1 genome. The promoter sequences of the BHV-1 thymidine kinase (tk), gB, gC, SV40 early, and pseudorabies virus (PRV) immediate early (IE) genes were placed at the upstream of the open reading frame of the chloramphenycol acetyl transferase (CAT) gene and the promoter-CAT sequences were integrated into the tk gene of BHV-1 by homologous recombination. The promoter activity was assayed by measuring the CAT activity in the extracts of Madin Darby bovine kidney (MDBK) cells infected with the recombinant BHV-1. The PRV IE promoter was activated earlier and maintained at a higher level activity than the BHV-1 gB or gC promoters throughout the most of the growth phase of BHV-1. At the late phase, however, the activities of the BHV-1 gB and gC promoters reached the higher level. The BHV-1 tk promoter activity was low and the SV40 early promoter was hardly activated when integrated into the BHV-1 genome.",
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Characterization of Promoters Integrated in the Genome of Bovine Herpesvirus-1 (BHV-1). / Murata, Takayuki; Xuan, Xuenan; Otsuka, Haruki.

In: Journal of Veterinary Medical Science, Vol. 61, No. 5, 01.05.1999, p. 453-457.

Research output: Contribution to journalArticle

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