Characterization of pulmonary carbonyl reductase of mouse and guinea pig

Toshihiro Nakayama, Koji Yashiro, Yoshio Inoue, Kazuya Matsuura, Hideshi Ichikawa, Akira Hara, Hideo Sawada

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Abstract

Carbonyl reductases were purified from mouse and guinea pig lung. The mouse enzyme exhibited structural and catalytic similarity to the guinea pig enzyme: tetrameric structure consisting of an identical 23 kDa subunit; basicity (pI of 8.8); low substrate specificity for alphatic and aromatic carbonyl compounds; dual cofactor specificity for NADPH and NADH; stereospecific transfer of the 4-pro S hydrogen of NADPH; and sensitivity to pyrazole, 2-mercaptoethanol and ferrous ion. Although 3-ketosteroids were extensively reduced by the mouse enzyme but not by the guinea pig enzyme in the forward reaction, the two enzymes similarly oxidized some alicyclic alcohols such as acenaphthenol, cyclohex-2-en-1-ol and benzenedihydrodiol in the presence of NADP+ and NAD+. A partial similarity between the two enzymes was observed immunologically, using antibodies against the purified guinea pig enzyme. The lung enzymes differ in several aspects from other oxidoreductases from extrapulmonary tissues. The immunoreactive protein was detected only in lung of the tissues of the two species.

Original languageEnglish
Pages (from-to)220-227
Number of pages8
JournalBBA - General Subjects
Volume882
Issue number2
DOIs
Publication statusPublished - 19-06-1986
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

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  • Cite this

    Nakayama, T., Yashiro, K., Inoue, Y., Matsuura, K., Ichikawa, H., Hara, A., & Sawada, H. (1986). Characterization of pulmonary carbonyl reductase of mouse and guinea pig. BBA - General Subjects, 882(2), 220-227. https://doi.org/10.1016/0304-4165(86)90158-3