Characterization of spontaneous excitatory synaptic currents in newt retinal bipolar cells

Fusao Kawai

doi:10.1016/S0304-3940(99)00511-X

Characterization of spontaneous excitatory synaptic currents in newt retinal bipolar cells

Fusao Kawai

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The kinetics of glutamate concentration in the synaptic cleft is an important determinant of synaptic function. To elucidate peak concentration of glutamate released from a single vesicle in the cleft, spontaneous excitatory postsynaptic currents (sEPSCs)in Off-bipolar cells from the sliced newt retina were analyzed using whole-cell patch clamp recording and the computer simulation. The sEPSCs were blocked by an AMPA/kainate (KA) antagonist, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), and prolonged by cyclothiazide. However, an N-methyl-D-aspartate (NMDA) antagonist, D-2- amino-5-phosphonopentanoic acid (D-AP5), was ineffective. These suggest that sEPSCs in Off-bipolar cells are mediated exclusively by AMPA/KA receptors, sEPSCs simulated by a detailed kinetic model of AMPA receptor best approximated the data, when peak glutamate concentration was 10 μM. Therefore, it was concluded that peak concentration of glutamate released from a single vesicle would be elevated to approximately 10 μM at the newt Off-bipolar dendrite.

Original language	English
Pages (from-to)	49-52
Number of pages	4
Journal	Neuroscience Letters
Volume	271
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3940(99)00511-X
Publication status	Published - 13-08-1999
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Neuroscience

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10.1016/S0304-3940(99)00511-X

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title = "Characterization of spontaneous excitatory synaptic currents in newt retinal bipolar cells",

abstract = "The kinetics of glutamate concentration in the synaptic cleft is an important determinant of synaptic function. To elucidate peak concentration of glutamate released from a single vesicle in the cleft, spontaneous excitatory postsynaptic currents (sEPSCs)in Off-bipolar cells from the sliced newt retina were analyzed using whole-cell patch clamp recording and the computer simulation. The sEPSCs were blocked by an AMPA/kainate (KA) antagonist, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), and prolonged by cyclothiazide. However, an N-methyl-D-aspartate (NMDA) antagonist, D-2- amino-5-phosphonopentanoic acid (D-AP5), was ineffective. These suggest that sEPSCs in Off-bipolar cells are mediated exclusively by AMPA/KA receptors, sEPSCs simulated by a detailed kinetic model of AMPA receptor best approximated the data, when peak glutamate concentration was 10 μM. Therefore, it was concluded that peak concentration of glutamate released from a single vesicle would be elevated to approximately 10 μM at the newt Off-bipolar dendrite.",

author = "Fusao Kawai",

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AU - Kawai, Fusao

PY - 1999/8/13

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N2 - The kinetics of glutamate concentration in the synaptic cleft is an important determinant of synaptic function. To elucidate peak concentration of glutamate released from a single vesicle in the cleft, spontaneous excitatory postsynaptic currents (sEPSCs)in Off-bipolar cells from the sliced newt retina were analyzed using whole-cell patch clamp recording and the computer simulation. The sEPSCs were blocked by an AMPA/kainate (KA) antagonist, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), and prolonged by cyclothiazide. However, an N-methyl-D-aspartate (NMDA) antagonist, D-2- amino-5-phosphonopentanoic acid (D-AP5), was ineffective. These suggest that sEPSCs in Off-bipolar cells are mediated exclusively by AMPA/KA receptors, sEPSCs simulated by a detailed kinetic model of AMPA receptor best approximated the data, when peak glutamate concentration was 10 μM. Therefore, it was concluded that peak concentration of glutamate released from a single vesicle would be elevated to approximately 10 μM at the newt Off-bipolar dendrite.

AB - The kinetics of glutamate concentration in the synaptic cleft is an important determinant of synaptic function. To elucidate peak concentration of glutamate released from a single vesicle in the cleft, spontaneous excitatory postsynaptic currents (sEPSCs)in Off-bipolar cells from the sliced newt retina were analyzed using whole-cell patch clamp recording and the computer simulation. The sEPSCs were blocked by an AMPA/kainate (KA) antagonist, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), and prolonged by cyclothiazide. However, an N-methyl-D-aspartate (NMDA) antagonist, D-2- amino-5-phosphonopentanoic acid (D-AP5), was ineffective. These suggest that sEPSCs in Off-bipolar cells are mediated exclusively by AMPA/KA receptors, sEPSCs simulated by a detailed kinetic model of AMPA receptor best approximated the data, when peak glutamate concentration was 10 μM. Therefore, it was concluded that peak concentration of glutamate released from a single vesicle would be elevated to approximately 10 μM at the newt Off-bipolar dendrite.

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Characterization of spontaneous excitatory synaptic currents in newt retinal bipolar cells

Abstract

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