Characterization of the cell growth analysis for detection of immortal cellular impurities in human mesenchymal stem cells

Ken Kono, Nozomi Takada, Satoshi Yasuda, Rumi Sawada, Shingo Niimi, Akifumi Matsuyama, Yoji Sato

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The analysis of invitro cell senescence/growth after serial passaging can be one of ways to show the absence of immortalized cells, which are frequently tumorigenic, in human cell-processed therapeutic products (hCTPs). However, the performance of the cell growth analysis for detection of the immortalized cellular impurities has never been evaluated. In the present study, we examined the growth rates of human mesenchymal stem cells (hMSCs, passage 5 (. P=5)) contaminated with various doses of HeLa cells, and compared with that of hMSCs alone. The growth rates of the contaminated hMSCs were comparable to that of hMSCs alone at P=5, but significantly increased at P=6 (0.1% and 0.01% HeLa) or P=7 (0.001% HeLa) within 30 days. These findings suggest that the cell growth analysis is a simple and sensitive method to detect immortalized cellular impurities in hCTPs derived from human somatic cells.

Original languageEnglish
Pages (from-to)146-149
Number of pages4
JournalBiologicals
Volume43
Issue number2
DOIs
Publication statusPublished - 01-03-2015

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Immunology and Microbiology(all)
  • Pharmacology

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