TY - JOUR
T1 - Characterization of the cell growth analysis for detection of immortal cellular impurities in human mesenchymal stem cells
AU - Kono, Ken
AU - Takada, Nozomi
AU - Yasuda, Satoshi
AU - Sawada, Rumi
AU - Niimi, Shingo
AU - Matsuyama, Akifumi
AU - Sato, Yoji
N1 - Publisher Copyright:
© 2014 The Authors.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - The analysis of invitro cell senescence/growth after serial passaging can be one of ways to show the absence of immortalized cells, which are frequently tumorigenic, in human cell-processed therapeutic products (hCTPs). However, the performance of the cell growth analysis for detection of the immortalized cellular impurities has never been evaluated. In the present study, we examined the growth rates of human mesenchymal stem cells (hMSCs, passage 5 (. P=5)) contaminated with various doses of HeLa cells, and compared with that of hMSCs alone. The growth rates of the contaminated hMSCs were comparable to that of hMSCs alone at P=5, but significantly increased at P=6 (0.1% and 0.01% HeLa) or P=7 (0.001% HeLa) within 30 days. These findings suggest that the cell growth analysis is a simple and sensitive method to detect immortalized cellular impurities in hCTPs derived from human somatic cells.
AB - The analysis of invitro cell senescence/growth after serial passaging can be one of ways to show the absence of immortalized cells, which are frequently tumorigenic, in human cell-processed therapeutic products (hCTPs). However, the performance of the cell growth analysis for detection of the immortalized cellular impurities has never been evaluated. In the present study, we examined the growth rates of human mesenchymal stem cells (hMSCs, passage 5 (. P=5)) contaminated with various doses of HeLa cells, and compared with that of hMSCs alone. The growth rates of the contaminated hMSCs were comparable to that of hMSCs alone at P=5, but significantly increased at P=6 (0.1% and 0.01% HeLa) or P=7 (0.001% HeLa) within 30 days. These findings suggest that the cell growth analysis is a simple and sensitive method to detect immortalized cellular impurities in hCTPs derived from human somatic cells.
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U2 - 10.1016/j.biologicals.2014.11.007
DO - 10.1016/j.biologicals.2014.11.007
M3 - Article
C2 - 25523786
AN - SCOPUS:84924070950
SN - 1045-1056
VL - 43
SP - 146
EP - 149
JO - Biologicals
JF - Biologicals
IS - 2
ER -