Characterization of the human DNA polymerase delta catalytic subunit expressed by a recombinant baculovirus.

S. Suzuki, Motoshi Suzuki, S. Yoshida

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The catalytic subunit of human DNA polymerase (pol) delta, p125, was expressed in recombinant baculovirus-infected insect cells, separated from a baculovirus-encoded DNA polymerase, and was purified to homogeneity by affinity trapping with a histidine-octapeptide at the C-terminus of p125 as the ligand. Purified p125 showed DNA polymerase activity resembling conventionally purified calf thymus pol delta. However, the two differed in four ways: 1) the specific activity of recombinant p125 was one quarter of the calf thymus pol delta; 2) the recombinant p125 was relatively resistant to aphidicolin; 3) the apparent Km for dTTP of the recombinant p125 was estimated at 33 microM, 15-fold the value for calf thymus pol delta; and 4) the recombinant p125 was not stimulated by recombinant PCNA, while activity of calf thymus pol delta increased 150-fold in response. Furthermore, PCNA did not stimulate either the p125 incubated with p50, a small subunit of pol delta, or co-expressed with p50 in insect cells. The full length recombinant p125 migrated slightly faster than pol delta from human cell lines, Jurkat or HeLa, upon SDS-polyacrylamide gel electrophoresis, suggesting a post-translational modification. The results indicate that in vivo assembly of the fully active complex of pol delta requires factors in addition to p125 and p50 subunits, and/or a post-translational modification of p125.

Original languageEnglish
Pages (from-to)99-113
Number of pages15
JournalNagoya journal of medical science
Volume63
Issue number3-4
Publication statusPublished - 01-01-2000
Externally publishedYes

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DNA Polymerase III
Baculoviridae
Thymus Gland
Catalytic Domain
Proliferating Cell Nuclear Antigen
DNA-Directed DNA Polymerase
Post Translational Protein Processing
Insects
Aphidicolin
Somatostatin-Secreting Cells
Histidine
Polyacrylamide Gel Electrophoresis
Ligands
Cell Line

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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abstract = "The catalytic subunit of human DNA polymerase (pol) delta, p125, was expressed in recombinant baculovirus-infected insect cells, separated from a baculovirus-encoded DNA polymerase, and was purified to homogeneity by affinity trapping with a histidine-octapeptide at the C-terminus of p125 as the ligand. Purified p125 showed DNA polymerase activity resembling conventionally purified calf thymus pol delta. However, the two differed in four ways: 1) the specific activity of recombinant p125 was one quarter of the calf thymus pol delta; 2) the recombinant p125 was relatively resistant to aphidicolin; 3) the apparent Km for dTTP of the recombinant p125 was estimated at 33 microM, 15-fold the value for calf thymus pol delta; and 4) the recombinant p125 was not stimulated by recombinant PCNA, while activity of calf thymus pol delta increased 150-fold in response. Furthermore, PCNA did not stimulate either the p125 incubated with p50, a small subunit of pol delta, or co-expressed with p50 in insect cells. The full length recombinant p125 migrated slightly faster than pol delta from human cell lines, Jurkat or HeLa, upon SDS-polyacrylamide gel electrophoresis, suggesting a post-translational modification. The results indicate that in vivo assembly of the fully active complex of pol delta requires factors in addition to p125 and p50 subunits, and/or a post-translational modification of p125.",
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Characterization of the human DNA polymerase delta catalytic subunit expressed by a recombinant baculovirus. / Suzuki, S.; Suzuki, Motoshi; Yoshida, S.

In: Nagoya journal of medical science, Vol. 63, No. 3-4, 01.01.2000, p. 99-113.

Research output: Contribution to journalArticle

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