Characterization of the human NTAK gene structure and distribution of the isoforms for rat NTAK mRNA

Kouji Yamada, Naohiro Ichino, Kazuhiro Nishii, Hirohide Sawada, Shigeki Higashiyama, Hiroshi Ishiguro, Toshiharu Nagatsu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

NTAK (neural- and thymus-derived activator for the ErbB kinase, neuregulin-2) is a novel member of the epidermal growth factor (EGF) family. We have isolated and characterized the human NTAK gene, comprising 12 exons spanning in excess of 55 kilobases (kb). The 7.0 kb long mRNA of the human NTAK gene was expressed in the human neuroblastoma SK-N-SH cell line with two alternative isoforms detected. Furthermore, six isoforms have been identified from rat brain and PC-12 cells. Although the α isoform of the NTAK gene was found to be expressed in all tissues including brain, the β isoform was expressed only in rat brain tissues. Potential regulatory regions included consensus binding sites for AP-2, TF-IIIA, Sp-1, and YY-1 located in the 5'- flanking region of the NTAK gene. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)15-24
Number of pages10
JournalGene
Volume255
Issue number1
DOIs
Publication statusPublished - 05-09-2000

Fingerprint

Protein Isoforms
Messenger RNA
Genes
Brain
Neuregulins
5' Flanking Region
Nucleic Acid Regulatory Sequences
Neuroblastoma
Epidermal Growth Factor
Thymus Gland
Exons
Phosphotransferases
Binding Sites
Cell Line

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Yamada, Kouji ; Ichino, Naohiro ; Nishii, Kazuhiro ; Sawada, Hirohide ; Higashiyama, Shigeki ; Ishiguro, Hiroshi ; Nagatsu, Toshiharu. / Characterization of the human NTAK gene structure and distribution of the isoforms for rat NTAK mRNA. In: Gene. 2000 ; Vol. 255, No. 1. pp. 15-24.
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Characterization of the human NTAK gene structure and distribution of the isoforms for rat NTAK mRNA. / Yamada, Kouji; Ichino, Naohiro; Nishii, Kazuhiro; Sawada, Hirohide; Higashiyama, Shigeki; Ishiguro, Hiroshi; Nagatsu, Toshiharu.

In: Gene, Vol. 255, No. 1, 05.09.2000, p. 15-24.

Research output: Contribution to journalArticle

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