Characterization of the neurotoxin produced by isolates associated with avian botulism

Masato Takeda, Kentaro Tsukamoto, Tomoko Kohda, Miki Matsui, Masafumi Mukamoto, Shunji Kozaki

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Several varieties of birds are affected by type C botulism. We conducted neutralization tests of culture supernatants of isolates from cases of avian botulism. Whereas the toxin produced by isolates derived from mammalian botulism was neutralized only with type C antitoxin, the toxins of all isolates related to avian botulism were neutralized with both type C and D antitoxins. An analysis of nucleotide sequences with several strains revealed that the neurotoxin gene in the isolates from avian botulism comprises two thirds of the type C neurotoxin gene and one third of the type D neurotoxin gene. This indicates that the neurotoxin of avian isolates is a mosaic of type C and D neurotoxins. We prepared three sets of primers to differentiate the gene for the mosaic form from the conserved genes of type C and D neurotoxins. The results of polymerase chain reaction with these primers indicated that all avian botulism-related isolates and specimens possess the gene for the mosaic form of the neurotoxin. The toxins purified from avian and mammalian isolates exhibited the same degree of lethality in mice, but the former showed greater toxicity to chickens than the latter. These results indicate that the mosaic neurotoxin is probably a pathogenic agent causing some forms of avian botulism.

Original languageEnglish
Pages (from-to)376-381
Number of pages6
JournalAvian Diseases
Volume49
Issue number3
DOIs
Publication statusPublished - 01-01-2005

Fingerprint

Botulism
botulism
neurotoxins
Neurotoxins
Genes
Antitoxins
antitoxins
toxins
genes
Neutralization Tests
neutralization tests
Birds
Chickens
polymerase chain reaction
Polymerase Chain Reaction
chickens
toxicity
nucleotide sequences
birds
mice

All Science Journal Classification (ASJC) codes

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

Cite this

Takeda, Masato ; Tsukamoto, Kentaro ; Kohda, Tomoko ; Matsui, Miki ; Mukamoto, Masafumi ; Kozaki, Shunji. / Characterization of the neurotoxin produced by isolates associated with avian botulism. In: Avian Diseases. 2005 ; Vol. 49, No. 3. pp. 376-381.
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Characterization of the neurotoxin produced by isolates associated with avian botulism. / Takeda, Masato; Tsukamoto, Kentaro; Kohda, Tomoko; Matsui, Miki; Mukamoto, Masafumi; Kozaki, Shunji.

In: Avian Diseases, Vol. 49, No. 3, 01.01.2005, p. 376-381.

Research output: Contribution to journalArticle

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