Abstract
The emergence and rapid spread of novel DS-1-like intergenogroup reassortant rotaviruses having the equine-like G3 genotype (DS-1-like G3P[8] strains) have been recently reported from several countries. During rotavirus surveillance in Japan in 2015–2016, three DS-1-like G3P[8] strains were identified from children with severe diarrhea. In the present study, we sequenced and characterized the full genomes of these three strains. On full-genomic analysis, all three strains showed a unique genotype constellation including both genogroup 1 and 2 genes: G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis revealed that each of the 11 genes of the three strains was closely related to that of Japanese DS-1-like G1P[8] and/or Japanese equine-like G3P[4] human strains. Thus, the three study strains were suggested to be reassortants that acquired the G3-VP7 gene from equine G3 rotaviruses on the genetic background of DS-1-like G1P[8] strains. Our observations will provide important insights into the evolutionary dynamics of emerging DS-1-like G3P[8] strains.
| Original language | English |
|---|---|
| Pages (from-to) | 890-898 |
| Number of pages | 9 |
| Journal | Journal of Medical Virology |
| Volume | 90 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 01-05-2018 |
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All Science Journal Classification (ASJC) codes
- Virology
- Infectious Diseases
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Characterization of unusual DS-1-like G3P[8] rotavirus strains in children with diarrhea in Japan. / Komoto, Satoshi; Ide, Tomihiko; Negoro, Manami; Tanaka, Takaaki; Asada, Kazutoyo; Umemoto, Masakazu; Kuroki, Haruo; Ito, Hiroaki; Tanaka, Shigeki; Ito, Mitsue; Fukuda, Saori; Suga, Shigeru; Kamiya, Hajime; Nakano, Takashi; Taniguchi, Koki.
In: Journal of Medical Virology, Vol. 90, No. 5, 01.05.2018, p. 890-898.Research output: Contribution to journal › Article
TY - JOUR
T1 - Characterization of unusual DS-1-like G3P[8] rotavirus strains in children with diarrhea in Japan
AU - Komoto, Satoshi
AU - Ide, Tomihiko
AU - Negoro, Manami
AU - Tanaka, Takaaki
AU - Asada, Kazutoyo
AU - Umemoto, Masakazu
AU - Kuroki, Haruo
AU - Ito, Hiroaki
AU - Tanaka, Shigeki
AU - Ito, Mitsue
AU - Fukuda, Saori
AU - Suga, Shigeru
AU - Kamiya, Hajime
AU - Nakano, Takashi
AU - Taniguchi, Koki
PY - 2018/5/1
Y1 - 2018/5/1
N2 - The emergence and rapid spread of novel DS-1-like intergenogroup reassortant rotaviruses having the equine-like G3 genotype (DS-1-like G3P[8] strains) have been recently reported from several countries. During rotavirus surveillance in Japan in 2015–2016, three DS-1-like G3P[8] strains were identified from children with severe diarrhea. In the present study, we sequenced and characterized the full genomes of these three strains. On full-genomic analysis, all three strains showed a unique genotype constellation including both genogroup 1 and 2 genes: G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis revealed that each of the 11 genes of the three strains was closely related to that of Japanese DS-1-like G1P[8] and/or Japanese equine-like G3P[4] human strains. Thus, the three study strains were suggested to be reassortants that acquired the G3-VP7 gene from equine G3 rotaviruses on the genetic background of DS-1-like G1P[8] strains. Our observations will provide important insights into the evolutionary dynamics of emerging DS-1-like G3P[8] strains.
AB - The emergence and rapid spread of novel DS-1-like intergenogroup reassortant rotaviruses having the equine-like G3 genotype (DS-1-like G3P[8] strains) have been recently reported from several countries. During rotavirus surveillance in Japan in 2015–2016, three DS-1-like G3P[8] strains were identified from children with severe diarrhea. In the present study, we sequenced and characterized the full genomes of these three strains. On full-genomic analysis, all three strains showed a unique genotype constellation including both genogroup 1 and 2 genes: G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis revealed that each of the 11 genes of the three strains was closely related to that of Japanese DS-1-like G1P[8] and/or Japanese equine-like G3P[4] human strains. Thus, the three study strains were suggested to be reassortants that acquired the G3-VP7 gene from equine G3 rotaviruses on the genetic background of DS-1-like G1P[8] strains. Our observations will provide important insights into the evolutionary dynamics of emerging DS-1-like G3P[8] strains.
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U2 - 10.1002/jmv.25016
DO - 10.1002/jmv.25016
M3 - Article
AN - SCOPUS:85041600375
VL - 90
SP - 890
EP - 898
JO - Journal of Medical Virology
JF - Journal of Medical Virology
SN - 0146-6615
IS - 5
ER -