Characterization of unusual DS-1-like G3P[8] rotavirus strains in children with diarrhea in Japan

Satoshi Komoto, Tomihiko Ide, Manami Negoro, Takaaki Tanaka, Kazutoyo Asada, Masakazu Umemoto, Haruo Kuroki, Hiroaki Ito, Shigeki Tanaka, Mitsue Ito, Saori Fukuda, Shigeru Suga, Hajime Kamiya, Takashi Nakano, Koki Taniguchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The emergence and rapid spread of novel DS-1-like intergenogroup reassortant rotaviruses having the equine-like G3 genotype (DS-1-like G3P[8] strains) have been recently reported from several countries. During rotavirus surveillance in Japan in 2015–2016, three DS-1-like G3P[8] strains were identified from children with severe diarrhea. In the present study, we sequenced and characterized the full genomes of these three strains. On full-genomic analysis, all three strains showed a unique genotype constellation including both genogroup 1 and 2 genes: G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis revealed that each of the 11 genes of the three strains was closely related to that of Japanese DS-1-like G1P[8] and/or Japanese equine-like G3P[4] human strains. Thus, the three study strains were suggested to be reassortants that acquired the G3-VP7 gene from equine G3 rotaviruses on the genetic background of DS-1-like G1P[8] strains. Our observations will provide important insights into the evolutionary dynamics of emerging DS-1-like G3P[8] strains.

Original languageEnglish
Pages (from-to)890-898
Number of pages9
JournalJournal of Medical Virology
Volume90
Issue number5
DOIs
Publication statusPublished - 01-05-2018

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Rotavirus
Horses
Diarrhea
Japan
Genotype
Genes
varespladib methyl
Genome

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

Komoto, Satoshi ; Ide, Tomihiko ; Negoro, Manami ; Tanaka, Takaaki ; Asada, Kazutoyo ; Umemoto, Masakazu ; Kuroki, Haruo ; Ito, Hiroaki ; Tanaka, Shigeki ; Ito, Mitsue ; Fukuda, Saori ; Suga, Shigeru ; Kamiya, Hajime ; Nakano, Takashi ; Taniguchi, Koki. / Characterization of unusual DS-1-like G3P[8] rotavirus strains in children with diarrhea in Japan. In: Journal of Medical Virology. 2018 ; Vol. 90, No. 5. pp. 890-898.
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abstract = "The emergence and rapid spread of novel DS-1-like intergenogroup reassortant rotaviruses having the equine-like G3 genotype (DS-1-like G3P[8] strains) have been recently reported from several countries. During rotavirus surveillance in Japan in 2015–2016, three DS-1-like G3P[8] strains were identified from children with severe diarrhea. In the present study, we sequenced and characterized the full genomes of these three strains. On full-genomic analysis, all three strains showed a unique genotype constellation including both genogroup 1 and 2 genes: G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis revealed that each of the 11 genes of the three strains was closely related to that of Japanese DS-1-like G1P[8] and/or Japanese equine-like G3P[4] human strains. Thus, the three study strains were suggested to be reassortants that acquired the G3-VP7 gene from equine G3 rotaviruses on the genetic background of DS-1-like G1P[8] strains. Our observations will provide important insights into the evolutionary dynamics of emerging DS-1-like G3P[8] strains.",
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Komoto, S, Ide, T, Negoro, M, Tanaka, T, Asada, K, Umemoto, M, Kuroki, H, Ito, H, Tanaka, S, Ito, M, Fukuda, S, Suga, S, Kamiya, H, Nakano, T & Taniguchi, K 2018, 'Characterization of unusual DS-1-like G3P[8] rotavirus strains in children with diarrhea in Japan', Journal of Medical Virology, vol. 90, no. 5, pp. 890-898. https://doi.org/10.1002/jmv.25016

Characterization of unusual DS-1-like G3P[8] rotavirus strains in children with diarrhea in Japan. / Komoto, Satoshi; Ide, Tomihiko; Negoro, Manami; Tanaka, Takaaki; Asada, Kazutoyo; Umemoto, Masakazu; Kuroki, Haruo; Ito, Hiroaki; Tanaka, Shigeki; Ito, Mitsue; Fukuda, Saori; Suga, Shigeru; Kamiya, Hajime; Nakano, Takashi; Taniguchi, Koki.

In: Journal of Medical Virology, Vol. 90, No. 5, 01.05.2018, p. 890-898.

Research output: Contribution to journalArticle

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T1 - Characterization of unusual DS-1-like G3P[8] rotavirus strains in children with diarrhea in Japan

AU - Komoto, Satoshi

AU - Ide, Tomihiko

AU - Negoro, Manami

AU - Tanaka, Takaaki

AU - Asada, Kazutoyo

AU - Umemoto, Masakazu

AU - Kuroki, Haruo

AU - Ito, Hiroaki

AU - Tanaka, Shigeki

AU - Ito, Mitsue

AU - Fukuda, Saori

AU - Suga, Shigeru

AU - Kamiya, Hajime

AU - Nakano, Takashi

AU - Taniguchi, Koki

PY - 2018/5/1

Y1 - 2018/5/1

N2 - The emergence and rapid spread of novel DS-1-like intergenogroup reassortant rotaviruses having the equine-like G3 genotype (DS-1-like G3P[8] strains) have been recently reported from several countries. During rotavirus surveillance in Japan in 2015–2016, three DS-1-like G3P[8] strains were identified from children with severe diarrhea. In the present study, we sequenced and characterized the full genomes of these three strains. On full-genomic analysis, all three strains showed a unique genotype constellation including both genogroup 1 and 2 genes: G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis revealed that each of the 11 genes of the three strains was closely related to that of Japanese DS-1-like G1P[8] and/or Japanese equine-like G3P[4] human strains. Thus, the three study strains were suggested to be reassortants that acquired the G3-VP7 gene from equine G3 rotaviruses on the genetic background of DS-1-like G1P[8] strains. Our observations will provide important insights into the evolutionary dynamics of emerging DS-1-like G3P[8] strains.

AB - The emergence and rapid spread of novel DS-1-like intergenogroup reassortant rotaviruses having the equine-like G3 genotype (DS-1-like G3P[8] strains) have been recently reported from several countries. During rotavirus surveillance in Japan in 2015–2016, three DS-1-like G3P[8] strains were identified from children with severe diarrhea. In the present study, we sequenced and characterized the full genomes of these three strains. On full-genomic analysis, all three strains showed a unique genotype constellation including both genogroup 1 and 2 genes: G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis revealed that each of the 11 genes of the three strains was closely related to that of Japanese DS-1-like G1P[8] and/or Japanese equine-like G3P[4] human strains. Thus, the three study strains were suggested to be reassortants that acquired the G3-VP7 gene from equine G3 rotaviruses on the genetic background of DS-1-like G1P[8] strains. Our observations will provide important insights into the evolutionary dynamics of emerging DS-1-like G3P[8] strains.

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