Characterizing CGI-94 (comparative gene identification-94) which is down-regulated in the hippocampus of early stage Alzheimer's disease brain

Klaus Heese; Takahiro Nakayama; Ryuji Hata; Makoto Masumura; Hiroyasu Akatsu; Feng Li; Yasuo Nagai; Takayuki Yamamoto; Kenji Kosaka; Takahiro Suemoto; Tohru Sawada

doi:10.1046/j.0953-816x.2001.01836.x

Characterizing CGI-94 (comparative gene identification-94) which is down-regulated in the hippocampus of early stage Alzheimer's disease brain

Klaus Heese, Takahiro Nakayama, Ryuji Hata, Makoto Masumura, Hiroyasu Akatsu, Feng Li, Yasuo Nagai, Takayuki Yamamoto, Kenji Kosaka, Takahiro Suemoto, Tohru Sawada

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The treatment of Alzheimer's disease (AD) remains a major challenge because of the incomplete understanding of the triggering events that lead to the selective neurodegeneration characteristic of AD brains. Here we describe a new protein, CGI-94, that is down-regulated at the mRNA level in the hippocampus of early stage AD brain. Transfection experiments with CGI-94 as a green florescent protein (GFP)-fusion-protein show that this protein is translocated into the nucleus of the cell. The finding that this protein, which has a bipartite nuclear localization signal, is also observed in the cytoplasm and extracellular space points to a multifunctional protein. Immunohistochemical analyses reveal that CGI-94 is mainly expressed in neurons of the hippocampal formation and the cortex but not in the cerebellar nucleus. In conclusion, the expression of the nucleolar phosphoprotein CGI-94 appears to be disturbed in early processes of neuronal degeneration.

Original language	English
Pages (from-to)	79-86
Number of pages	8
Journal	European Journal of Neuroscience
Volume	15
Issue number	1
DOIs	https://doi.org/10.1046/j.0953-816x.2001.01836.x
Publication status	Published - 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Neuroscience

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10.1046/j.0953-816x.2001.01836.x

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Heese, K., Nakayama, T., Hata, R., Masumura, M., Akatsu, H., Li, F., Nagai, Y., Yamamoto, T., Kosaka, K., Suemoto, T., & Sawada, T. (2002). Characterizing CGI-94 (comparative gene identification-94) which is down-regulated in the hippocampus of early stage Alzheimer's disease brain. European Journal of Neuroscience, 15(1), 79-86. https://doi.org/10.1046/j.0953-816x.2001.01836.x

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title = "Characterizing CGI-94 (comparative gene identification-94) which is down-regulated in the hippocampus of early stage Alzheimer's disease brain",

abstract = "The treatment of Alzheimer's disease (AD) remains a major challenge because of the incomplete understanding of the triggering events that lead to the selective neurodegeneration characteristic of AD brains. Here we describe a new protein, CGI-94, that is down-regulated at the mRNA level in the hippocampus of early stage AD brain. Transfection experiments with CGI-94 as a green florescent protein (GFP)-fusion-protein show that this protein is translocated into the nucleus of the cell. The finding that this protein, which has a bipartite nuclear localization signal, is also observed in the cytoplasm and extracellular space points to a multifunctional protein. Immunohistochemical analyses reveal that CGI-94 is mainly expressed in neurons of the hippocampal formation and the cortex but not in the cerebellar nucleus. In conclusion, the expression of the nucleolar phosphoprotein CGI-94 appears to be disturbed in early processes of neuronal degeneration.",

keywords = "Amyloid β-protein, Neurodegeneration, Neurotoxicity, Neurotrophic factor",

author = "Klaus Heese and Takahiro Nakayama and Ryuji Hata and Makoto Masumura and Hiroyasu Akatsu and Feng Li and Yasuo Nagai and Takayuki Yamamoto and Kenji Kosaka and Takahiro Suemoto and Tohru Sawada",

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Heese, K, Nakayama, T, Hata, R, Masumura, M, Akatsu, H, Li, F, Nagai, Y, Yamamoto, T, Kosaka, K, Suemoto, T & Sawada, T 2002, 'Characterizing CGI-94 (comparative gene identification-94) which is down-regulated in the hippocampus of early stage Alzheimer's disease brain', European Journal of Neuroscience, vol. 15, no. 1, pp. 79-86. https://doi.org/10.1046/j.0953-816x.2001.01836.x

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AU - Heese, Klaus

AU - Nakayama, Takahiro

AU - Hata, Ryuji

AU - Masumura, Makoto

AU - Akatsu, Hiroyasu

AU - Li, Feng

AU - Nagai, Yasuo

AU - Yamamoto, Takayuki

AU - Kosaka, Kenji

AU - Suemoto, Takahiro

AU - Sawada, Tohru

PY - 2002

Y1 - 2002

N2 - The treatment of Alzheimer's disease (AD) remains a major challenge because of the incomplete understanding of the triggering events that lead to the selective neurodegeneration characteristic of AD brains. Here we describe a new protein, CGI-94, that is down-regulated at the mRNA level in the hippocampus of early stage AD brain. Transfection experiments with CGI-94 as a green florescent protein (GFP)-fusion-protein show that this protein is translocated into the nucleus of the cell. The finding that this protein, which has a bipartite nuclear localization signal, is also observed in the cytoplasm and extracellular space points to a multifunctional protein. Immunohistochemical analyses reveal that CGI-94 is mainly expressed in neurons of the hippocampal formation and the cortex but not in the cerebellar nucleus. In conclusion, the expression of the nucleolar phosphoprotein CGI-94 appears to be disturbed in early processes of neuronal degeneration.

AB - The treatment of Alzheimer's disease (AD) remains a major challenge because of the incomplete understanding of the triggering events that lead to the selective neurodegeneration characteristic of AD brains. Here we describe a new protein, CGI-94, that is down-regulated at the mRNA level in the hippocampus of early stage AD brain. Transfection experiments with CGI-94 as a green florescent protein (GFP)-fusion-protein show that this protein is translocated into the nucleus of the cell. The finding that this protein, which has a bipartite nuclear localization signal, is also observed in the cytoplasm and extracellular space points to a multifunctional protein. Immunohistochemical analyses reveal that CGI-94 is mainly expressed in neurons of the hippocampal formation and the cortex but not in the cerebellar nucleus. In conclusion, the expression of the nucleolar phosphoprotein CGI-94 appears to be disturbed in early processes of neuronal degeneration.

KW - Amyloid β-protein

KW - Neurodegeneration

KW - Neurotoxicity

KW - Neurotrophic factor

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SN - 0953-816X

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JF - European Journal of Neuroscience

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ER -

Characterizing CGI-94 (comparative gene identification-94) which is down-regulated in the hippocampus of early stage Alzheimer's disease brain

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