Chemotactic behavior of egg mitochondria in response to sperm fusion in mice

Maki Iwai; Yuichirou Harada; Rinako Miyabayashi; Woojin Kang; Akihiro Nakamura; Natsuko Kawano; Yoshitaka Miyamoto; Mitsutoshi Yamada; Toshio Hamatani; Mami Miyado; Keiichi Yoshida; Hidekazu Saito; Mamoru Tanaka; Akihiro Umezawa; Kenji Miyado

doi:10.1016/j.heliyon.2018.e00944

Chemotactic behavior of egg mitochondria in response to sperm fusion in mice

Maki Iwai, Yuichirou Harada, Rinako Miyabayashi, Woojin Kang, Akihiro Nakamura, Natsuko Kawano, Yoshitaka Miyamoto, Mitsutoshi Yamada, Toshio Hamatani, Mami Miyado, Keiichi Yoshida, Hidekazu Saito, Mamoru Tanaka, Akihiro Umezawa, Kenji Miyado

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Mitochondria are the powerhouses of eukaryotic cells and their positioning contributes to fertilization and early developmental processes. We report that sperm fusion triggers Ca²⁺ oscillations and mitochondrial movement toward fused sperm (mitochondrial chemotaxis) in mouse eggs. Mitochondria functioned in Ca²⁺ storage and were colocalized with endoplasmic reticulum (ER) during Ca²⁺ oscillations. Mitochondria then moved toward the fused sperm. Sperm extracts lacking nuclei induced Ca²⁺ oscillations, but did not promote mitochondrial chemotaxis. Our results suggest that sperm fusion motivates Ca²⁺ oscillation-independent mitochondrial chemotaxis. This phenomenon indicates that egg mitochondria interact with sperm materials, presumably nuclear substances, and their network tethers egg and sperm nuclei at the early stage of zygote formation.

Original language	English
Article number	e00944
Journal	Heliyon
Volume	4
Issue number	11
DOIs	https://doi.org/10.1016/j.heliyon.2018.e00944
Publication status	Published - 11-2018
Externally published	Yes

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title = "Chemotactic behavior of egg mitochondria in response to sperm fusion in mice",

abstract = "Mitochondria are the powerhouses of eukaryotic cells and their positioning contributes to fertilization and early developmental processes. We report that sperm fusion triggers Ca2+ oscillations and mitochondrial movement toward fused sperm (mitochondrial chemotaxis) in mouse eggs. Mitochondria functioned in Ca2+ storage and were colocalized with endoplasmic reticulum (ER) during Ca2+ oscillations. Mitochondria then moved toward the fused sperm. Sperm extracts lacking nuclei induced Ca2+ oscillations, but did not promote mitochondrial chemotaxis. Our results suggest that sperm fusion motivates Ca2+ oscillation-independent mitochondrial chemotaxis. This phenomenon indicates that egg mitochondria interact with sperm materials, presumably nuclear substances, and their network tethers egg and sperm nuclei at the early stage of zygote formation.",

keywords = "Cell biology, Developmental biology, Molecular biology, Zoology",

author = "Maki Iwai and Yuichirou Harada and Rinako Miyabayashi and Woojin Kang and Akihiro Nakamura and Natsuko Kawano and Yoshitaka Miyamoto and Mitsutoshi Yamada and Toshio Hamatani and Mami Miyado and Keiichi Yoshida and Hidekazu Saito and Mamoru Tanaka and Akihiro Umezawa and Kenji Miyado",

note = "Publisher Copyright: {\textcopyright} 2018 The Authors",

year = "2018",

month = nov,

doi = "10.1016/j.heliyon.2018.e00944",

language = "English",

volume = "4",

journal = "Heliyon",

issn = "2405-8440",

publisher = "Elsevier Ltd",

number = "11",

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TY - JOUR

T1 - Chemotactic behavior of egg mitochondria in response to sperm fusion in mice

AU - Iwai, Maki

AU - Harada, Yuichirou

AU - Miyabayashi, Rinako

AU - Kang, Woojin

AU - Nakamura, Akihiro

AU - Kawano, Natsuko

AU - Miyamoto, Yoshitaka

AU - Yamada, Mitsutoshi

AU - Hamatani, Toshio

AU - Miyado, Mami

AU - Yoshida, Keiichi

AU - Saito, Hidekazu

AU - Tanaka, Mamoru

AU - Umezawa, Akihiro

AU - Miyado, Kenji

PY - 2018/11

Y1 - 2018/11

N2 - Mitochondria are the powerhouses of eukaryotic cells and their positioning contributes to fertilization and early developmental processes. We report that sperm fusion triggers Ca2+ oscillations and mitochondrial movement toward fused sperm (mitochondrial chemotaxis) in mouse eggs. Mitochondria functioned in Ca2+ storage and were colocalized with endoplasmic reticulum (ER) during Ca2+ oscillations. Mitochondria then moved toward the fused sperm. Sperm extracts lacking nuclei induced Ca2+ oscillations, but did not promote mitochondrial chemotaxis. Our results suggest that sperm fusion motivates Ca2+ oscillation-independent mitochondrial chemotaxis. This phenomenon indicates that egg mitochondria interact with sperm materials, presumably nuclear substances, and their network tethers egg and sperm nuclei at the early stage of zygote formation.

AB - Mitochondria are the powerhouses of eukaryotic cells and their positioning contributes to fertilization and early developmental processes. We report that sperm fusion triggers Ca2+ oscillations and mitochondrial movement toward fused sperm (mitochondrial chemotaxis) in mouse eggs. Mitochondria functioned in Ca2+ storage and were colocalized with endoplasmic reticulum (ER) during Ca2+ oscillations. Mitochondria then moved toward the fused sperm. Sperm extracts lacking nuclei induced Ca2+ oscillations, but did not promote mitochondrial chemotaxis. Our results suggest that sperm fusion motivates Ca2+ oscillation-independent mitochondrial chemotaxis. This phenomenon indicates that egg mitochondria interact with sperm materials, presumably nuclear substances, and their network tethers egg and sperm nuclei at the early stage of zygote formation.

KW - Cell biology

KW - Developmental biology

KW - Molecular biology

KW - Zoology

UR - http://www.scopus.com/inward/record.url?scp=85056624673&partnerID=8YFLogxK

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U2 - 10.1016/j.heliyon.2018.e00944

DO - 10.1016/j.heliyon.2018.e00944

M3 - Article

AN - SCOPUS:85056624673

SN - 2405-8440

VL - 4

JO - Heliyon

JF - Heliyon

IS - 11

M1 - e00944

ER -

Chemotactic behavior of egg mitochondria in response to sperm fusion in mice

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