Cholesterol uptake of isolated rat hepatocytes is accelerated by several kinds of phosphatidylcholine

Yoichi Nagamura, Kunio Sasaki, Madoka Ito, Kuniaki Saito, Naomichi Ogitsu, Isao Ishiguro

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Abstract

Uptake of cholesterol by isolated rat hepatocytes in a serum-free medium was remarkably enchanced by dispersion with several kinds of phosphatidylcholine. Of the various phosphatidylcholines tested, dilinoylphosphatidylcholine had the strongest accelerating effect, while dipalmitoylphosphatidylcholine was the weakest. The abilities to accelerate cholesterol uptake were in proportion to the content of unsaturated fatty acid in the phosphatidylcholine used. It was confirmed by electron microscopy that there is no relation between the size of the cholesterol-phosphatidylcholine complex and uptake. These data suggest that recognition of unsaturated fatty acids in phosphatidylcholine by isolated cells enhances uptake of cholesterol. Cholesterol uptake (Isolated rat hepatocyte) Phosphatidylcholine Unsaturated fatty acid Dioleoylphosphatidylcholine Dipalmitoylphosphatidylcholine.

Original languageEnglish
Pages (from-to)91-94
Number of pages4
JournalFEBS Letters
Volume203
Issue number1
DOIs
Publication statusPublished - 14-07-1986

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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