Abstract
Chromosomal aberrations have been thought to be random events. However, recent findings introduce a new paradigm in which certain DNA segments have the potential to adopt unusual conformations that lead to genomic instability and nonrandom chromosomal rearrangement. One of the best-studied examples is the palindromic AT-rich repeat (PATRR), which induces recurrent constitutional translocations in humans. Here, we established a plasmid-based model that promotes frequent intermolecular rearrangements between two PATRRs in HEK293 cells. In this model system, the proportion of PATRR plasmid that extrudes a cruciform structure correlates to the levels of rearrangement. Our data suggest that PATRR-mediated translocations are attributable to unusual DNA conformations that confer a common pathway for chromosomal rearrangements in humans.
Original language | English |
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Pages (from-to) | 191-198 |
Number of pages | 8 |
Journal | Genome Research |
Volume | 19 |
Issue number | 2 |
DOIs | |
Publication status | Published - 02-2009 |
All Science Journal Classification (ASJC) codes
- Genetics
- Genetics(clinical)