TY - JOUR
T1 - Circadian rhythms of sorting nexin 25 in the mouse suprachiasmatic nucleus
AU - Takemura, Shoko
AU - Nagano, Mamoru
AU - Isonishi, Ayami
AU - Tanaka, Tatsuhide
AU - Tatsumi, Kouko
AU - Yamano, Mariko
AU - Minami, Yoichi
AU - Shigeyoshi, Yasufumi
AU - Wanaka, Akio
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/5/14
Y1 - 2020/5/14
N2 - Entrainment of mammalian circadian rhythms requires receptor-mediated signaling in the hypothalamic suprachiasmatic nucleus (SCN), the site of the master circadian pacemaker. Receptor-mediated signaling is regulated by endocytosis, indicating that endocytosis-related proteins contribute to SCN pacemaking. Sorting nexin 25 (SNX25) belongs to the sorting nexin superfamily, whose members are responsible for membrane attachment to organelles of the endocytic system. In this study, we showed that Snx25 mRNA and SNX25 protein are highly expressed and exhibit remarkable circadian rhythms in the SCN of adult mice. Expression was maximal at about zeitgeber time (ZT) 16 in the subjective night and minimal at ZT8 in the subjective day. Prominent SNX25 immunoreactivity was found in the arginine vasopressin-positive neurons of the SCN. These findings suggest that SNX25 is a new actor in endocytic signaling, perhaps contributing to the circadian pacemaking system.
AB - Entrainment of mammalian circadian rhythms requires receptor-mediated signaling in the hypothalamic suprachiasmatic nucleus (SCN), the site of the master circadian pacemaker. Receptor-mediated signaling is regulated by endocytosis, indicating that endocytosis-related proteins contribute to SCN pacemaking. Sorting nexin 25 (SNX25) belongs to the sorting nexin superfamily, whose members are responsible for membrane attachment to organelles of the endocytic system. In this study, we showed that Snx25 mRNA and SNX25 protein are highly expressed and exhibit remarkable circadian rhythms in the SCN of adult mice. Expression was maximal at about zeitgeber time (ZT) 16 in the subjective night and minimal at ZT8 in the subjective day. Prominent SNX25 immunoreactivity was found in the arginine vasopressin-positive neurons of the SCN. These findings suggest that SNX25 is a new actor in endocytic signaling, perhaps contributing to the circadian pacemaking system.
KW - Endocytosis
KW - G protein-coupled receptor (GPCR)
KW - Regulator of G protein signaling (RGS)
KW - Rhythmic expression
UR - https://www.scopus.com/pages/publications/85082857617
UR - https://www.scopus.com/pages/publications/85082857617#tab=citedBy
U2 - 10.1016/j.neulet.2020.134897
DO - 10.1016/j.neulet.2020.134897
M3 - Article
C2 - 32156614
AN - SCOPUS:85082857617
SN - 0304-3940
VL - 727
JO - Neuroscience Letters
JF - Neuroscience Letters
M1 - 134897
ER -