Circuit-dependent striatal PKA and ERK signaling underlies rapid behavioral shift in mating reaction of male mice

Akihiro Goto, Ichiro Nakahara, Takashi Yamaguchi, Yuji Kamioka, Kenta Sumiyama, Michiyuki Matsuda, Shigetada Nakanishi, Kazuo Funabiki

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The selection of reward-seeking and aversive behaviors is controlled by two distinct D1 and D2 receptor-expressing striatal medium spiny neurons, namely the direct pathway MSNs (dMSNs) and the indirect pathway MSNs (iMSNs), but the dynamic modulation of signaling cascades of dMSNs and iMSNs in behaving animals remains largely elusive. We developed an in vivo methodology to monitor Förster resonance energy transfer (FRET) of the activities of PKA and ERK in either dMSNs or iMSNs by microendoscopy in freely moving mice. PKA and ERK were coordinately but oppositely regulated between dMSNs and iMSNs by rewarding cocaine administration and aversive electric shocks. Notably, the activities of PKA and ERK rapidly shifted when male mice became active or indifferent toward female mice during mating behavior. Importantly, manipulation of PKA cascades by the Designer Receptor recapitulated active and indifferent mating behaviors, indicating a causal linkage of a dynamic activity shift of PKA and ERK between dMSNs and iMSNs in action selection.

Original languageEnglish
Pages (from-to)6718-6723
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number21
DOIs
Publication statusPublished - 26-05-2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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