Cis-acting effect of the IL1B C-31T polymorphism on IL-1β mRNA expression

R. Kimura, T. Nishioka, A. Soemantri, T. Ishida

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

Since the effect of IL1B polymorphisms on IL-1β production is still controversial, we selected two polymorphisms to test their cis-acting effect on IL-1β mRNA expression by means of the allele-specific transcript quantification and the haplotype analysis. As for the C-31T polymorphism, we found that expression of the -31T allele was 2.2 times of the -31C allele. This higher transcription efficiency may correspond to the fact that C-31T is located in a TATA box. The other polymorphism, C+3954T, did not alter the levels of transcription. The use of the allele-specific transcript quantification enables us to exclude trans-acting effects of polymorphisms on the gene expression and contributes to understanding the roles of the IL1B polymorphisms in susceptibility to multifactorial diseases.

Original languageEnglish
Pages (from-to)572-575
Number of pages4
JournalGenes and Immunity
Volume5
Issue number7
DOIs
Publication statusPublished - 11-2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics
  • Genetics(clinical)

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