Classical MHC class I genes composed of highly divergent sequence lineages share a single locus in rainbow trout (Oncorhynchus mykiss)

K. Aoyagi, J・m Dijkstra, C. Xia, I. Denda, M. Ototake, Keiichiro Hashimoto, T. Nakanishi

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The classical MHC class I genes have been known to be highly polymorphic in various vertebrates. To date, putative allelic sequences of the classical MHC class I genes in teleost fish have been reported in several studies. However, the establishment of their allelic status has been hampered in most cases by the lack of appropriate genomic information. In the present study, using heterozygous and homozygous fish, we obtained classical-type MHC class I sequences of rainbow trout (Oncorhynchus mykiss) and investigated their allelic relationship by gene amplification and Southern and Northern hybridization analyses. The results indicated that all MHC class I sequences we obtained were derived from a single locus. Based on this, a unique polymorphic nature of the MHC class I locus of rainbow trout has been revealed. The mosaic combination of highly divergent ancient sequences in the peptide-binding domains is notable, and the variable nature around the boundary between the α3 and transmembrane domains is unprecedented.

Original languageEnglish
Pages (from-to)260-273
Number of pages14
JournalJournal of Immunology
Volume168
Issue number1
DOIs
Publication statusPublished - 01-01-2002

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MHC Class I Genes
Oncorhynchus mykiss
Fishes
Gene Amplification
Vertebrates

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

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abstract = "The classical MHC class I genes have been known to be highly polymorphic in various vertebrates. To date, putative allelic sequences of the classical MHC class I genes in teleost fish have been reported in several studies. However, the establishment of their allelic status has been hampered in most cases by the lack of appropriate genomic information. In the present study, using heterozygous and homozygous fish, we obtained classical-type MHC class I sequences of rainbow trout (Oncorhynchus mykiss) and investigated their allelic relationship by gene amplification and Southern and Northern hybridization analyses. The results indicated that all MHC class I sequences we obtained were derived from a single locus. Based on this, a unique polymorphic nature of the MHC class I locus of rainbow trout has been revealed. The mosaic combination of highly divergent ancient sequences in the peptide-binding domains is notable, and the variable nature around the boundary between the α3 and transmembrane domains is unprecedented.",
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Classical MHC class I genes composed of highly divergent sequence lineages share a single locus in rainbow trout (Oncorhynchus mykiss). / Aoyagi, K.; Dijkstra, J・m; Xia, C.; Denda, I.; Ototake, M.; Hashimoto, Keiichiro; Nakanishi, T.

In: Journal of Immunology, Vol. 168, No. 1, 01.01.2002, p. 260-273.

Research output: Contribution to journalArticle

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AU - Aoyagi, K.

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AU - Xia, C.

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AU - Hashimoto, Keiichiro

AU - Nakanishi, T.

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