Cleavage of carcinoembryonic antigen induces metastatic potential in colorectal carcinoma

Yasuko Yamamoto, Eiichiro Hirakawa, Seiji Mori, Yoshinosuke Hamada, Naomasa Kawaguchi, Nariaki Matsuura

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Carcinoembryonic antigen (CEA), a widely used tumor marker, is attached by a glycosylphosphatidylinositol (GPI) anchor motif to the cell membrane. Recent study suggested that membrane-bound CEA might be cleaved by glycosylphosphatidylinositol-phospholipase D (GPI-PLD). We studied the effect of GPI-PLD on the cleavage of CEA to elucidate the implication for metastatic potential in colorectal carcinoma cells. CEA amount of conditioned medium was changed by suramin and phenanthroline (activator and inhibitor of GPI-PLD) only in SW620 and SW837 which expressed both CEA and GPI-PLD mRNA. Suramin treatment also augmented migratory activity and decreased cell surface CEA expression in SW620 and SW837. Furthermore, GPI-PLD knockdown cells using GPI-PLD-specific siRNA in SW620 and SW837 showed decreased CEA secretion from cell membrane and the migration activity, increased membrane-bound CEA amount. Splenic injection of SW620 and SW837 induced marked hepatic metastases in nude mice. These results suggest that membrane-bound CEA is cleaved by GPI-PLD and that this cleavage enhances the metastatic potential in colorectal carcinoma cells.

Original languageEnglish
Pages (from-to)223-229
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume333
Issue number1
DOIs
Publication statusPublished - 22-07-2005
Externally publishedYes

Fingerprint

Carcinoembryonic Antigen
Glycosylphosphatidylinositols
Colorectal Neoplasms
Suramin
Cell membranes
Membranes
Cells
Cell Membrane
Phospholipase D
Phenanthrolines
Surface Antigens
Tumor Biomarkers
Conditioned Culture Medium
Nude Mice
Small Interfering RNA
Cell Movement
1-dodecylpyridoxal
Neoplasm Metastasis
Messenger RNA
Injections

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yamamoto, Yasuko ; Hirakawa, Eiichiro ; Mori, Seiji ; Hamada, Yoshinosuke ; Kawaguchi, Naomasa ; Matsuura, Nariaki. / Cleavage of carcinoembryonic antigen induces metastatic potential in colorectal carcinoma. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 333, No. 1. pp. 223-229.
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Cleavage of carcinoembryonic antigen induces metastatic potential in colorectal carcinoma. / Yamamoto, Yasuko; Hirakawa, Eiichiro; Mori, Seiji; Hamada, Yoshinosuke; Kawaguchi, Naomasa; Matsuura, Nariaki.

In: Biochemical and Biophysical Research Communications, Vol. 333, No. 1, 22.07.2005, p. 223-229.

Research output: Contribution to journalArticle

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