Clinical, biochemical and molecular analysis of 13 Japanese patients with β-ureidopropionase deficiency demonstrates high prevalence of the c.977G > A (p.R326Q) mutation

Yoko Nakajima, Judith Meijer, Doreen Dobritzsch, Tetsuya Ito, Rutger Meinsma, Nico G.G.M. Abeling, Jeroen Roelofsen, Lida Zoetekouw, Yoriko Watanabe, Kyoko Tashiro, Tomoko Lee, Yasuhiro Takeshima, Hiroshi Mitsubuchi, Akira Yoneyama, Kazuhide Ohta, Kaoru Eto, Kayoko Saito, Tomiko Kuhara, André B.P. van Kuilenburg

Research output: Contribution to journalArticle

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Abstract

β-ureidopropionase (βUP) deficiency is an autosomal recessive disease characterized by N-carbamyl-β-amino aciduria. To date, only 16 genetically confirmed patients with βUP deficiency have been reported. Here, we report on the clinical, biochemical and molecular findings of 13 Japanese βUP deficient patients. In this group of patients, three novel missense mutations (p.G31S, p.E271K, and p.I286T) and a recently described mutation (p.R326Q) were identified. The p.R326Q mutation was detected in all 13 patients with eight patients being homozygous for this mutation. Screening for the p.R326Q mutation in 110 Japanese individuals showed an allele frequency of 0.9 %. Transient expression of mutant βUP enzymes in HEK293 cells showed that the p.E271K and p.R326Q mutations cause profound decreases in activity (≤ 1.3 %). Conversely, βUP enzymes containing the p.G31S and p.I286T mutations possess residual activities of 50 and 70 %, respectively, suggesting we cannot exclude the presence of additional mutations in the non-coding region of the UPB1 gene. Analysis of a human βUP homology model revealed that the effects of the mutations (p.G31S, p.E271K, and p.R326Q) on enzyme activity are most likely linked to improper oligomer assembly. Highly variable phenotypes ranging from neurological involvement (including convulsions and autism) to asymptomatic, were observed in diagnosed patients. High prevalence of p.R326Q in the normal Japanese population indicates that βUP deficiency is not as rare as generally considered and screening for βUP deficiency should be included in diagnosis of patients with unexplained neurological abnormalities.

Original languageEnglish
Pages (from-to)801-812
Number of pages12
JournalJournal of Inherited Metabolic Disease
Volume37
Issue number5
DOIs
Publication statusPublished - 01-09-2014
Externally publishedYes

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Mutation
Enzymes
HEK293 Cells
Missense Mutation
Autistic Disorder
Gene Frequency
Seizures
Phenotype
Population
Genes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

Nakajima, Yoko ; Meijer, Judith ; Dobritzsch, Doreen ; Ito, Tetsuya ; Meinsma, Rutger ; Abeling, Nico G.G.M. ; Roelofsen, Jeroen ; Zoetekouw, Lida ; Watanabe, Yoriko ; Tashiro, Kyoko ; Lee, Tomoko ; Takeshima, Yasuhiro ; Mitsubuchi, Hiroshi ; Yoneyama, Akira ; Ohta, Kazuhide ; Eto, Kaoru ; Saito, Kayoko ; Kuhara, Tomiko ; van Kuilenburg, André B.P. / Clinical, biochemical and molecular analysis of 13 Japanese patients with β-ureidopropionase deficiency demonstrates high prevalence of the c.977G > A (p.R326Q) mutation. In: Journal of Inherited Metabolic Disease. 2014 ; Vol. 37, No. 5. pp. 801-812.
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abstract = "β-ureidopropionase (βUP) deficiency is an autosomal recessive disease characterized by N-carbamyl-β-amino aciduria. To date, only 16 genetically confirmed patients with βUP deficiency have been reported. Here, we report on the clinical, biochemical and molecular findings of 13 Japanese βUP deficient patients. In this group of patients, three novel missense mutations (p.G31S, p.E271K, and p.I286T) and a recently described mutation (p.R326Q) were identified. The p.R326Q mutation was detected in all 13 patients with eight patients being homozygous for this mutation. Screening for the p.R326Q mutation in 110 Japanese individuals showed an allele frequency of 0.9 {\%}. Transient expression of mutant βUP enzymes in HEK293 cells showed that the p.E271K and p.R326Q mutations cause profound decreases in activity (≤ 1.3 {\%}). Conversely, βUP enzymes containing the p.G31S and p.I286T mutations possess residual activities of 50 and 70 {\%}, respectively, suggesting we cannot exclude the presence of additional mutations in the non-coding region of the UPB1 gene. Analysis of a human βUP homology model revealed that the effects of the mutations (p.G31S, p.E271K, and p.R326Q) on enzyme activity are most likely linked to improper oligomer assembly. Highly variable phenotypes ranging from neurological involvement (including convulsions and autism) to asymptomatic, were observed in diagnosed patients. High prevalence of p.R326Q in the normal Japanese population indicates that βUP deficiency is not as rare as generally considered and screening for βUP deficiency should be included in diagnosis of patients with unexplained neurological abnormalities.",
author = "Yoko Nakajima and Judith Meijer and Doreen Dobritzsch and Tetsuya Ito and Rutger Meinsma and Abeling, {Nico G.G.M.} and Jeroen Roelofsen and Lida Zoetekouw and Yoriko Watanabe and Kyoko Tashiro and Tomoko Lee and Yasuhiro Takeshima and Hiroshi Mitsubuchi and Akira Yoneyama and Kazuhide Ohta and Kaoru Eto and Kayoko Saito and Tomiko Kuhara and {van Kuilenburg}, {Andr{\'e} B.P.}",
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Nakajima, Y, Meijer, J, Dobritzsch, D, Ito, T, Meinsma, R, Abeling, NGGM, Roelofsen, J, Zoetekouw, L, Watanabe, Y, Tashiro, K, Lee, T, Takeshima, Y, Mitsubuchi, H, Yoneyama, A, Ohta, K, Eto, K, Saito, K, Kuhara, T & van Kuilenburg, ABP 2014, 'Clinical, biochemical and molecular analysis of 13 Japanese patients with β-ureidopropionase deficiency demonstrates high prevalence of the c.977G > A (p.R326Q) mutation', Journal of Inherited Metabolic Disease, vol. 37, no. 5, pp. 801-812. https://doi.org/10.1007/s10545-014-9682-y

Clinical, biochemical and molecular analysis of 13 Japanese patients with β-ureidopropionase deficiency demonstrates high prevalence of the c.977G > A (p.R326Q) mutation. / Nakajima, Yoko; Meijer, Judith; Dobritzsch, Doreen; Ito, Tetsuya; Meinsma, Rutger; Abeling, Nico G.G.M.; Roelofsen, Jeroen; Zoetekouw, Lida; Watanabe, Yoriko; Tashiro, Kyoko; Lee, Tomoko; Takeshima, Yasuhiro; Mitsubuchi, Hiroshi; Yoneyama, Akira; Ohta, Kazuhide; Eto, Kaoru; Saito, Kayoko; Kuhara, Tomiko; van Kuilenburg, André B.P.

In: Journal of Inherited Metabolic Disease, Vol. 37, No. 5, 01.09.2014, p. 801-812.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Clinical, biochemical and molecular analysis of 13 Japanese patients with β-ureidopropionase deficiency demonstrates high prevalence of the c.977G > A (p.R326Q) mutation

AU - Nakajima, Yoko

AU - Meijer, Judith

AU - Dobritzsch, Doreen

AU - Ito, Tetsuya

AU - Meinsma, Rutger

AU - Abeling, Nico G.G.M.

AU - Roelofsen, Jeroen

AU - Zoetekouw, Lida

AU - Watanabe, Yoriko

AU - Tashiro, Kyoko

AU - Lee, Tomoko

AU - Takeshima, Yasuhiro

AU - Mitsubuchi, Hiroshi

AU - Yoneyama, Akira

AU - Ohta, Kazuhide

AU - Eto, Kaoru

AU - Saito, Kayoko

AU - Kuhara, Tomiko

AU - van Kuilenburg, André B.P.

PY - 2014/9/1

Y1 - 2014/9/1

N2 - β-ureidopropionase (βUP) deficiency is an autosomal recessive disease characterized by N-carbamyl-β-amino aciduria. To date, only 16 genetically confirmed patients with βUP deficiency have been reported. Here, we report on the clinical, biochemical and molecular findings of 13 Japanese βUP deficient patients. In this group of patients, three novel missense mutations (p.G31S, p.E271K, and p.I286T) and a recently described mutation (p.R326Q) were identified. The p.R326Q mutation was detected in all 13 patients with eight patients being homozygous for this mutation. Screening for the p.R326Q mutation in 110 Japanese individuals showed an allele frequency of 0.9 %. Transient expression of mutant βUP enzymes in HEK293 cells showed that the p.E271K and p.R326Q mutations cause profound decreases in activity (≤ 1.3 %). Conversely, βUP enzymes containing the p.G31S and p.I286T mutations possess residual activities of 50 and 70 %, respectively, suggesting we cannot exclude the presence of additional mutations in the non-coding region of the UPB1 gene. Analysis of a human βUP homology model revealed that the effects of the mutations (p.G31S, p.E271K, and p.R326Q) on enzyme activity are most likely linked to improper oligomer assembly. Highly variable phenotypes ranging from neurological involvement (including convulsions and autism) to asymptomatic, were observed in diagnosed patients. High prevalence of p.R326Q in the normal Japanese population indicates that βUP deficiency is not as rare as generally considered and screening for βUP deficiency should be included in diagnosis of patients with unexplained neurological abnormalities.

AB - β-ureidopropionase (βUP) deficiency is an autosomal recessive disease characterized by N-carbamyl-β-amino aciduria. To date, only 16 genetically confirmed patients with βUP deficiency have been reported. Here, we report on the clinical, biochemical and molecular findings of 13 Japanese βUP deficient patients. In this group of patients, three novel missense mutations (p.G31S, p.E271K, and p.I286T) and a recently described mutation (p.R326Q) were identified. The p.R326Q mutation was detected in all 13 patients with eight patients being homozygous for this mutation. Screening for the p.R326Q mutation in 110 Japanese individuals showed an allele frequency of 0.9 %. Transient expression of mutant βUP enzymes in HEK293 cells showed that the p.E271K and p.R326Q mutations cause profound decreases in activity (≤ 1.3 %). Conversely, βUP enzymes containing the p.G31S and p.I286T mutations possess residual activities of 50 and 70 %, respectively, suggesting we cannot exclude the presence of additional mutations in the non-coding region of the UPB1 gene. Analysis of a human βUP homology model revealed that the effects of the mutations (p.G31S, p.E271K, and p.R326Q) on enzyme activity are most likely linked to improper oligomer assembly. Highly variable phenotypes ranging from neurological involvement (including convulsions and autism) to asymptomatic, were observed in diagnosed patients. High prevalence of p.R326Q in the normal Japanese population indicates that βUP deficiency is not as rare as generally considered and screening for βUP deficiency should be included in diagnosis of patients with unexplained neurological abnormalities.

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