Clinical features and viral excretion in an infant with primary human herpesvirus 7 infection

Y. Asano, S. Suga, T. Yoshikawa, T. Yazaki, T. Uchikawa

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Objective. To find clinical features of a virologically-confirmed patient with primary human herpesvirus 7 (HHV-7) infection and a relationship of the excretion of viruses between HHV-7 and human herpesvirus 6 (HHV-6). Patient and Methods. A 13-month-old boy who had a known prior history of exanthem subitum at 6 months of age developed fever for 3 days and a skin rash appeared as the fever was resolving. The course was accompanying with nonspecific signs and symptoms such as anorexia, irritability, mild diarrhea, palpebral edema, mild inflammation of pharynx, and mild occipital and cervical lymphadenopathy. Heparinized blood samples were used for isolation of HHV-6 and HHV-7 and detection of both virus DNA sequences by a nested polymerase chain reaction (PCR) amplification. Samples from other body sites were also tested for their DNA sequences using the PCR. Both virus antibody activity was measured by an indirect immunofluorescent assay or a neutralization test. Results. Cultured mononuclear cells from the patient at the acute stage of the disease produced morphologic changes, which reacted only with the monoclonal antibody to HHV-7 but not with the antibody to HHV- 6. Both viruses were not isolated from blood obtained at the convalescent stage. An antibody response of the patient indicated a seroconversion to HHV- 7 but not to other microbial agents including HHV-6 and Mycoplasma pneumoniae. Both virus DNA sequences were detected in peripheral blood mononuclear cells at acute and convalescent stages. HHV-7 DNA was excreted into saliva and transiently into stool at an early convalescent stage followed by HHV-6 excretion into saliva. No HHV-7 and HHV-6 was excreted into urine. Conclusions. Clinical features of a virologically confirmed patient with primary HHV-7 infection were comparable with those of primary HHV-6 infection and HHV-7 infection may reactivate HHV-6.

Original languageEnglish
Pages (from-to)187-190
Number of pages4
JournalPediatrics
Volume95
Issue number2
Publication statusPublished - 01-01-1995

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Human Herpesvirus 7
Herpesviridae Infections
Human Herpesvirus 6
Viruses
Exanthema
Saliva
Fever
Neutralization Tests
Polymerase Chain Reaction
Mycoplasma pneumoniae
Antibodies
Anorexia
Acute Disease
Eyelids
Pharynx
Signs and Symptoms
Antibody Formation
Cultured Cells
Diarrhea
Edema

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health

Cite this

Asano, Y. ; Suga, S. ; Yoshikawa, T. ; Yazaki, T. ; Uchikawa, T. / Clinical features and viral excretion in an infant with primary human herpesvirus 7 infection. In: Pediatrics. 1995 ; Vol. 95, No. 2. pp. 187-190.
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Asano, Y, Suga, S, Yoshikawa, T, Yazaki, T & Uchikawa, T 1995, 'Clinical features and viral excretion in an infant with primary human herpesvirus 7 infection', Pediatrics, vol. 95, no. 2, pp. 187-190.

Clinical features and viral excretion in an infant with primary human herpesvirus 7 infection. / Asano, Y.; Suga, S.; Yoshikawa, T.; Yazaki, T.; Uchikawa, T.

In: Pediatrics, Vol. 95, No. 2, 01.01.1995, p. 187-190.

Research output: Contribution to journalArticle

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T1 - Clinical features and viral excretion in an infant with primary human herpesvirus 7 infection

AU - Asano, Y.

AU - Suga, S.

AU - Yoshikawa, T.

AU - Yazaki, T.

AU - Uchikawa, T.

PY - 1995/1/1

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N2 - Objective. To find clinical features of a virologically-confirmed patient with primary human herpesvirus 7 (HHV-7) infection and a relationship of the excretion of viruses between HHV-7 and human herpesvirus 6 (HHV-6). Patient and Methods. A 13-month-old boy who had a known prior history of exanthem subitum at 6 months of age developed fever for 3 days and a skin rash appeared as the fever was resolving. The course was accompanying with nonspecific signs and symptoms such as anorexia, irritability, mild diarrhea, palpebral edema, mild inflammation of pharynx, and mild occipital and cervical lymphadenopathy. Heparinized blood samples were used for isolation of HHV-6 and HHV-7 and detection of both virus DNA sequences by a nested polymerase chain reaction (PCR) amplification. Samples from other body sites were also tested for their DNA sequences using the PCR. Both virus antibody activity was measured by an indirect immunofluorescent assay or a neutralization test. Results. Cultured mononuclear cells from the patient at the acute stage of the disease produced morphologic changes, which reacted only with the monoclonal antibody to HHV-7 but not with the antibody to HHV- 6. Both viruses were not isolated from blood obtained at the convalescent stage. An antibody response of the patient indicated a seroconversion to HHV- 7 but not to other microbial agents including HHV-6 and Mycoplasma pneumoniae. Both virus DNA sequences were detected in peripheral blood mononuclear cells at acute and convalescent stages. HHV-7 DNA was excreted into saliva and transiently into stool at an early convalescent stage followed by HHV-6 excretion into saliva. No HHV-7 and HHV-6 was excreted into urine. Conclusions. Clinical features of a virologically confirmed patient with primary HHV-7 infection were comparable with those of primary HHV-6 infection and HHV-7 infection may reactivate HHV-6.

AB - Objective. To find clinical features of a virologically-confirmed patient with primary human herpesvirus 7 (HHV-7) infection and a relationship of the excretion of viruses between HHV-7 and human herpesvirus 6 (HHV-6). Patient and Methods. A 13-month-old boy who had a known prior history of exanthem subitum at 6 months of age developed fever for 3 days and a skin rash appeared as the fever was resolving. The course was accompanying with nonspecific signs and symptoms such as anorexia, irritability, mild diarrhea, palpebral edema, mild inflammation of pharynx, and mild occipital and cervical lymphadenopathy. Heparinized blood samples were used for isolation of HHV-6 and HHV-7 and detection of both virus DNA sequences by a nested polymerase chain reaction (PCR) amplification. Samples from other body sites were also tested for their DNA sequences using the PCR. Both virus antibody activity was measured by an indirect immunofluorescent assay or a neutralization test. Results. Cultured mononuclear cells from the patient at the acute stage of the disease produced morphologic changes, which reacted only with the monoclonal antibody to HHV-7 but not with the antibody to HHV- 6. Both viruses were not isolated from blood obtained at the convalescent stage. An antibody response of the patient indicated a seroconversion to HHV- 7 but not to other microbial agents including HHV-6 and Mycoplasma pneumoniae. Both virus DNA sequences were detected in peripheral blood mononuclear cells at acute and convalescent stages. HHV-7 DNA was excreted into saliva and transiently into stool at an early convalescent stage followed by HHV-6 excretion into saliva. No HHV-7 and HHV-6 was excreted into urine. Conclusions. Clinical features of a virologically confirmed patient with primary HHV-7 infection were comparable with those of primary HHV-6 infection and HHV-7 infection may reactivate HHV-6.

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