Clinical significance and problems in HCV measurement--comparison of CLEIA method with PCR method

Hirotoshi Ohta, Masao Takemura, Nobuyuki Furuta, Masumi Akiyama, Yasuo Katagiri, Hazuki Ohashi, Kanako Takahashi, Kuniaki Saito, Mitsuru Seishima

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Infection of a hepatitis C virus (HCV) is confirmed by the presence of HCV antibody or HCV-RNA. Recently, a highly sensitive method to examine HCV-core antigen has been developed. In this study, to evaluate the clinical significance of HCV-core antigen determination, we examined serum HCV infection markers, HCV-core antigen, HCV-RNA (AMPLICOR) and HCV-antibody (third generation) concentrations. We determined 225 serum samples, and three patients receiving the treatment with interferon. In 102 HCV-RNA positive samples, significant correlation was observed between HCV-RNA and HCV-core antigen (r=0.734, p<0.0001). However, three out of 102 (2.9%) cases were included within the negative range of HCV-core antigen (20 fmol/l). The HCV-core antigen value in three patients receiving the treatment with interferon paralleled with the amount of HCV-RNA. The determination of HCV-core antigen by CLEIA is a useful and time-saving method, but an attention should be paid to the presence of false-negative cases.

Original languageEnglish
Pages (from-to)813-818
Number of pages6
JournalRinsho byori. The Japanese journal of clinical pathology
Volume52
Issue number10
Publication statusPublished - 10-2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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    Ohta, H., Takemura, M., Furuta, N., Akiyama, M., Katagiri, Y., Ohashi, H., Takahashi, K., Saito, K., & Seishima, M. (2004). Clinical significance and problems in HCV measurement--comparison of CLEIA method with PCR method. Rinsho byori. The Japanese journal of clinical pathology, 52(10), 813-818.