Clinical significance of CD7‐positive stem cell leukemia. A distinct subtype of mixed lineage leukemia

Keiko Yumura‐Yagi, Junichi Hara, Hiroki Kurahashi, Jun Okamura, Shoichi Koizumi, Yasunori Toyoda, Norihide Murayama, Masami Inoue, Shigehiko Ishihara, Akio Tawa, Tetsuo Nishiura, Yoshio Kaneyama, Shintaro Okada, Keisei Kawa‐Ha

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Abstract

Ten leukemia cases with mixed phenotype were investigated in terms of clinical characteristics and cellular origin. Three patients were infants and six patients were older children. Six of them had a high leukocyte count and a mediastinal mass was found in three cases. All but one showed hepatosplenomegaly and/or lymphoadenopathy. In spite of intensive chemotherapy, most of them responded poorly. Cytochemical analysis of their leukemic cells revealed a low percentage of positivity for myeloperoxidase reactivity (< 25%) in two cases and electron microscopic platelet peroxidase reactivity was found in one of three analyzed cases. Phenotypically, these cells all expressed CD7, and other T‐lineage‐associated, B‐lineage‐associated, and/or myeloid‐associated antigens were also detected to some extent. In addition, three cases expressed CD41 and one case expressed CD56. The T‐cell receptor (TCR) genes and immunoglobulin gene were in the germline configuration in seven cases. In three rearranged cases two showed only the TCR‐δ gene rearrangement, and one had both TCR‐γ and δ gene rearrangements. Cell culture studies with 12–0‐tetradecanoyl‐phorbol‐13‐acetate (TPA) revealed differentiation to the T‐lineage in two cases and to a myeloid lineage in one case. Megakaryocytic differentiation was detected in two cases in culture without TPA. These results suggest that the cells from these cases arose from stem cells capable of both lymphoid and nonlymphoid differentiation. Although the cells were heterogeneous with regard to their potency of differentiation, they have similar clinical characteristics. Because of poor prognosis, it is important to identify this type of leukemia, and allogenic or autologous bone marrow transplantation should be considered. Cancer 68:2273–2280, 1991.

Original languageEnglish
Pages (from-to)2273-2280
Number of pages8
JournalCancer
Volume68
Issue number10
DOIs
Publication statusPublished - 01-01-1991
Externally publishedYes

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Leukemia
Stem Cells
Gene Rearrangement
Peroxidase
Immunoglobulin Genes
Autologous Transplantation
Bone Marrow Transplantation
Leukocyte Count
Blood Platelets
Cell Culture Techniques
Electrons
Phenotype
Antigens
Drug Therapy
Genes
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Yumura‐Yagi, Keiko ; Hara, Junichi ; Kurahashi, Hiroki ; Okamura, Jun ; Koizumi, Shoichi ; Toyoda, Yasunori ; Murayama, Norihide ; Inoue, Masami ; Ishihara, Shigehiko ; Tawa, Akio ; Nishiura, Tetsuo ; Kaneyama, Yoshio ; Okada, Shintaro ; Kawa‐Ha, Keisei. / Clinical significance of CD7‐positive stem cell leukemia. A distinct subtype of mixed lineage leukemia. In: Cancer. 1991 ; Vol. 68, No. 10. pp. 2273-2280.
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abstract = "Ten leukemia cases with mixed phenotype were investigated in terms of clinical characteristics and cellular origin. Three patients were infants and six patients were older children. Six of them had a high leukocyte count and a mediastinal mass was found in three cases. All but one showed hepatosplenomegaly and/or lymphoadenopathy. In spite of intensive chemotherapy, most of them responded poorly. Cytochemical analysis of their leukemic cells revealed a low percentage of positivity for myeloperoxidase reactivity (< 25{\%}) in two cases and electron microscopic platelet peroxidase reactivity was found in one of three analyzed cases. Phenotypically, these cells all expressed CD7, and other T‐lineage‐associated, B‐lineage‐associated, and/or myeloid‐associated antigens were also detected to some extent. In addition, three cases expressed CD41 and one case expressed CD56. The T‐cell receptor (TCR) genes and immunoglobulin gene were in the germline configuration in seven cases. In three rearranged cases two showed only the TCR‐δ gene rearrangement, and one had both TCR‐γ and δ gene rearrangements. Cell culture studies with 12–0‐tetradecanoyl‐phorbol‐13‐acetate (TPA) revealed differentiation to the T‐lineage in two cases and to a myeloid lineage in one case. Megakaryocytic differentiation was detected in two cases in culture without TPA. These results suggest that the cells from these cases arose from stem cells capable of both lymphoid and nonlymphoid differentiation. Although the cells were heterogeneous with regard to their potency of differentiation, they have similar clinical characteristics. Because of poor prognosis, it is important to identify this type of leukemia, and allogenic or autologous bone marrow transplantation should be considered. Cancer 68:2273–2280, 1991.",
author = "Keiko Yumura‐Yagi and Junichi Hara and Hiroki Kurahashi and Jun Okamura and Shoichi Koizumi and Yasunori Toyoda and Norihide Murayama and Masami Inoue and Shigehiko Ishihara and Akio Tawa and Tetsuo Nishiura and Yoshio Kaneyama and Shintaro Okada and Keisei Kawa‐Ha",
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Yumura‐Yagi, K, Hara, J, Kurahashi, H, Okamura, J, Koizumi, S, Toyoda, Y, Murayama, N, Inoue, M, Ishihara, S, Tawa, A, Nishiura, T, Kaneyama, Y, Okada, S & Kawa‐Ha, K 1991, 'Clinical significance of CD7‐positive stem cell leukemia. A distinct subtype of mixed lineage leukemia', Cancer, vol. 68, no. 10, pp. 2273-2280. https://doi.org/10.1002/1097-0142(19911115)68:10<2273::AID-CNCR2820681028>3.0.CO;2-G

Clinical significance of CD7‐positive stem cell leukemia. A distinct subtype of mixed lineage leukemia. / Yumura‐Yagi, Keiko; Hara, Junichi; Kurahashi, Hiroki; Okamura, Jun; Koizumi, Shoichi; Toyoda, Yasunori; Murayama, Norihide; Inoue, Masami; Ishihara, Shigehiko; Tawa, Akio; Nishiura, Tetsuo; Kaneyama, Yoshio; Okada, Shintaro; Kawa‐Ha, Keisei.

In: Cancer, Vol. 68, No. 10, 01.01.1991, p. 2273-2280.

Research output: Contribution to journalArticle

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T1 - Clinical significance of CD7‐positive stem cell leukemia. A distinct subtype of mixed lineage leukemia

AU - Yumura‐Yagi, Keiko

AU - Hara, Junichi

AU - Kurahashi, Hiroki

AU - Okamura, Jun

AU - Koizumi, Shoichi

AU - Toyoda, Yasunori

AU - Murayama, Norihide

AU - Inoue, Masami

AU - Ishihara, Shigehiko

AU - Tawa, Akio

AU - Nishiura, Tetsuo

AU - Kaneyama, Yoshio

AU - Okada, Shintaro

AU - Kawa‐Ha, Keisei

PY - 1991/1/1

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N2 - Ten leukemia cases with mixed phenotype were investigated in terms of clinical characteristics and cellular origin. Three patients were infants and six patients were older children. Six of them had a high leukocyte count and a mediastinal mass was found in three cases. All but one showed hepatosplenomegaly and/or lymphoadenopathy. In spite of intensive chemotherapy, most of them responded poorly. Cytochemical analysis of their leukemic cells revealed a low percentage of positivity for myeloperoxidase reactivity (< 25%) in two cases and electron microscopic platelet peroxidase reactivity was found in one of three analyzed cases. Phenotypically, these cells all expressed CD7, and other T‐lineage‐associated, B‐lineage‐associated, and/or myeloid‐associated antigens were also detected to some extent. In addition, three cases expressed CD41 and one case expressed CD56. The T‐cell receptor (TCR) genes and immunoglobulin gene were in the germline configuration in seven cases. In three rearranged cases two showed only the TCR‐δ gene rearrangement, and one had both TCR‐γ and δ gene rearrangements. Cell culture studies with 12–0‐tetradecanoyl‐phorbol‐13‐acetate (TPA) revealed differentiation to the T‐lineage in two cases and to a myeloid lineage in one case. Megakaryocytic differentiation was detected in two cases in culture without TPA. These results suggest that the cells from these cases arose from stem cells capable of both lymphoid and nonlymphoid differentiation. Although the cells were heterogeneous with regard to their potency of differentiation, they have similar clinical characteristics. Because of poor prognosis, it is important to identify this type of leukemia, and allogenic or autologous bone marrow transplantation should be considered. Cancer 68:2273–2280, 1991.

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