Clinical significance of major and minor bcr/abl chimeric transcripts in essential thrombocythemia

Hironori Yamada; Takaya Murakami; Tsuguhiro Kaneda; Mayumi Tadachi; Makoto Utsumi; Saburo Minami; Motohiro Hamaguchi; Masanobu Kasai; Yoshihisa Kodera; Haruhiko Ohashi; Yoshihisa Morishita; Teruhiko Terasawa; Yumiko Yamasaki; Yoshikazu Kamiya; Mamiko Hattori; Katsuo Yamanaka; Keitaro Tsushita; Masanori Shimoyama

doi:10.1093/jjco/hyd122

Clinical significance of major and minor bcr/abl chimeric transcripts in essential thrombocythemia

Hironori Yamada, Takaya Murakami, Tsuguhiro Kaneda, Mayumi Tadachi, Makoto Utsumi, Saburo Minami, Motohiro Hamaguchi, Masanobu Kasai, Yoshihisa Kodera, Haruhiko Ohashi, Yoshihisa Morishita, Teruhiko Terasawa, Yumiko Yamasaki, Yoshikazu Kamiya, Mamiko Hattori, Katsuo Yamanaka, Keitaro Tsushita, Masanori Shimoyama

Research output: Contribution to journal › Article › peer-review

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Abstract

Background: Contradictory results have been reported in terms of detecting bcr/abl transcripts in patients with essential thrombocythemia. The aim of this study was to investigate whether the bcr/abl transcript could be found in patients with essential thrombocythemia (ET). Methods: The bcr/abl transcript was amplified by the RT-nested PCR method using RNA extract from leukocytes taken from 14 essential thrombocythemia patients. The amplified DNAs were electrophoresed in 1% agarose and visualized with ethidium bromide. The DNA bands associated with the bcr/abl transcript were then extracted and followed by DNA sequence analysis. Results: Major bcr/abl transcripts of the b3a2 type and minor ones of the e1a2 type were found in one and two ET patients, respectively. The incidence of bcr/abl transcripts was 21.4% (three of 14 patients). Conclusion: Our experiments confirmed that bcr/abl transcripts are present in some patients with essential thrombocythemia.

Original language	English
Pages (from-to)	472-477
Number of pages	6
Journal	Japanese journal of clinical oncology
Volume	30
Issue number	11
DOIs	https://doi.org/10.1093/jjco/hyd122
Publication status	Published - 2000
Externally published	Yes

All Science Journal Classification (ASJC) codes

Oncology
Radiology Nuclear Medicine and imaging
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/jjco/hyd122

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Yamada, H., Murakami, T., Kaneda, T., Tadachi, M., Utsumi, M., Minami, S., Hamaguchi, M., Kasai, M., Kodera, Y., Ohashi, H., Morishita, Y., Terasawa, T., Yamasaki, Y., Kamiya, Y., Hattori, M., Yamanaka, K., Tsushita, K., & Shimoyama, M. (2000). Clinical significance of major and minor bcr/abl chimeric transcripts in essential thrombocythemia. Japanese journal of clinical oncology, 30(11), 472-477. https://doi.org/10.1093/jjco/hyd122

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title = "Clinical significance of major and minor bcr/abl chimeric transcripts in essential thrombocythemia",

abstract = "Background: Contradictory results have been reported in terms of detecting bcr/abl transcripts in patients with essential thrombocythemia. The aim of this study was to investigate whether the bcr/abl transcript could be found in patients with essential thrombocythemia (ET). Methods: The bcr/abl transcript was amplified by the RT-nested PCR method using RNA extract from leukocytes taken from 14 essential thrombocythemia patients. The amplified DNAs were electrophoresed in 1% agarose and visualized with ethidium bromide. The DNA bands associated with the bcr/abl transcript were then extracted and followed by DNA sequence analysis. Results: Major bcr/abl transcripts of the b3a2 type and minor ones of the e1a2 type were found in one and two ET patients, respectively. The incidence of bcr/abl transcripts was 21.4% (three of 14 patients). Conclusion: Our experiments confirmed that bcr/abl transcripts are present in some patients with essential thrombocythemia.",

author = "Hironori Yamada and Takaya Murakami and Tsuguhiro Kaneda and Mayumi Tadachi and Makoto Utsumi and Saburo Minami and Motohiro Hamaguchi and Masanobu Kasai and Yoshihisa Kodera and Haruhiko Ohashi and Yoshihisa Morishita and Teruhiko Terasawa and Yumiko Yamasaki and Yoshikazu Kamiya and Mamiko Hattori and Katsuo Yamanaka and Keitaro Tsushita and Masanori Shimoyama",

year = "2000",

doi = "10.1093/jjco/hyd122",

language = "English",

volume = "30",

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Yamada, H, Murakami, T, Kaneda, T, Tadachi, M, Utsumi, M, Minami, S, Hamaguchi, M, Kasai, M, Kodera, Y, Ohashi, H, Morishita, Y, Terasawa, T, Yamasaki, Y, Kamiya, Y, Hattori, M, Yamanaka, K, Tsushita, K & Shimoyama, M 2000, 'Clinical significance of major and minor bcr/abl chimeric transcripts in essential thrombocythemia', Japanese journal of clinical oncology, vol. 30, no. 11, pp. 472-477. https://doi.org/10.1093/jjco/hyd122

TY - JOUR

T1 - Clinical significance of major and minor bcr/abl chimeric transcripts in essential thrombocythemia

AU - Yamada, Hironori

AU - Murakami, Takaya

AU - Kaneda, Tsuguhiro

AU - Tadachi, Mayumi

AU - Utsumi, Makoto

AU - Minami, Saburo

AU - Hamaguchi, Motohiro

AU - Kasai, Masanobu

AU - Kodera, Yoshihisa

AU - Ohashi, Haruhiko

AU - Morishita, Yoshihisa

AU - Terasawa, Teruhiko

AU - Yamasaki, Yumiko

AU - Kamiya, Yoshikazu

AU - Hattori, Mamiko

AU - Yamanaka, Katsuo

AU - Tsushita, Keitaro

AU - Shimoyama, Masanori

PY - 2000

Y1 - 2000

N2 - Background: Contradictory results have been reported in terms of detecting bcr/abl transcripts in patients with essential thrombocythemia. The aim of this study was to investigate whether the bcr/abl transcript could be found in patients with essential thrombocythemia (ET). Methods: The bcr/abl transcript was amplified by the RT-nested PCR method using RNA extract from leukocytes taken from 14 essential thrombocythemia patients. The amplified DNAs were electrophoresed in 1% agarose and visualized with ethidium bromide. The DNA bands associated with the bcr/abl transcript were then extracted and followed by DNA sequence analysis. Results: Major bcr/abl transcripts of the b3a2 type and minor ones of the e1a2 type were found in one and two ET patients, respectively. The incidence of bcr/abl transcripts was 21.4% (three of 14 patients). Conclusion: Our experiments confirmed that bcr/abl transcripts are present in some patients with essential thrombocythemia.

AB - Background: Contradictory results have been reported in terms of detecting bcr/abl transcripts in patients with essential thrombocythemia. The aim of this study was to investigate whether the bcr/abl transcript could be found in patients with essential thrombocythemia (ET). Methods: The bcr/abl transcript was amplified by the RT-nested PCR method using RNA extract from leukocytes taken from 14 essential thrombocythemia patients. The amplified DNAs were electrophoresed in 1% agarose and visualized with ethidium bromide. The DNA bands associated with the bcr/abl transcript were then extracted and followed by DNA sequence analysis. Results: Major bcr/abl transcripts of the b3a2 type and minor ones of the e1a2 type were found in one and two ET patients, respectively. The incidence of bcr/abl transcripts was 21.4% (three of 14 patients). Conclusion: Our experiments confirmed that bcr/abl transcripts are present in some patients with essential thrombocythemia.

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DO - 10.1093/jjco/hyd122

M3 - Article

C2 - 11155916

AN - SCOPUS:0034524187

SN - 0368-2811

VL - 30

SP - 472

EP - 477

JO - Japanese journal of clinical oncology

JF - Japanese journal of clinical oncology

IS - 11

ER -

Clinical significance of major and minor bcr/abl chimeric transcripts in essential thrombocythemia

Abstract

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