Clinical significance of major and minor bcr/abl chimeric transcripts in essential thrombocythemia

Hironori Yamada, Takaya Murakami, Tsuguhiro Kaneda, Mayumi Tadachi, Makoto Utsumi, Saburo Minami, Motohiro Hamaguchi, Masanobu Kasai, Yoshihisa Kodera, Haruhiko Ohashi, Yoshihisa Morishita, Teruhiko Terasawa, Yumiko Yamasaki, Yoshikazu Kamiya, Mamiko Hattori, Katsuo Yamanaka, Keitaro Tsushita, Masanori Shimoyama

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Background: Contradictory results have been reported in terms of detecting bcr/abl transcripts in patients with essential thrombocythemia. The aim of this study was to investigate whether the bcr/abl transcript could be found in patients with essential thrombocythemia (ET). Methods: The bcr/abl transcript was amplified by the RT-nested PCR method using RNA extract from leukocytes taken from 14 essential thrombocythemia patients. The amplified DNAs were electrophoresed in 1% agarose and visualized with ethidium bromide. The DNA bands associated with the bcr/abl transcript were then extracted and followed by DNA sequence analysis. Results: Major bcr/abl transcripts of the b3a2 type and minor ones of the e1a2 type were found in one and two ET patients, respectively. The incidence of bcr/abl transcripts was 21.4% (three of 14 patients). Conclusion: Our experiments confirmed that bcr/abl transcripts are present in some patients with essential thrombocythemia.

Original languageEnglish
Pages (from-to)472-477
Number of pages6
JournalJapanese journal of clinical oncology
Volume30
Issue number11
DOIs
Publication statusPublished - 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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