Clinical significance of serum HMGB-1 and sRAGE levels in systemic sclerosis: Association with disease severity

Ayumi Yoshizaki, Kazuhiro Komura, Yohei Iwata, Fumihide Ogawa, Toshihide Hara, Eiji Muroi, Motoi Takenaka, Kazuhiro Shimizu, Minoru Hasegawa, Manabu Fujimoto, Shinichi Sato

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Introduction: The high mobility group box 1 protein (HMGB-1)/advanced glycation end products (RAGE) system is recently shown to play an important part in immune/inflammatory disorders. However, the association of this system in systemic sclerosis (SSc) remains unknown. Materials and Methods: To determine clinical association of serum levels of HMGB-1 and soluble RAGE (sRAGE) in patients with SSc, sera from 70 patients with SSc and 25 healthy controls were examined by enzyme-linked immunosorbent assay. Sera from tight-skin mice and bleomycin-induced scleroderma mice, animal models for SSc, were also examined. Skin HMGB-1 and RAGE expression was assessed by immunohistochemistry. Results and Discussion: Serum HMGB-1 and sRAGE levels in SSc were higher than those in controls. Similarly, HMGB-1 and sRAGE levels in animal SSc models were higher than those in control mice. SSc patients with elevated HMGB-1 and sRAGE levels had more frequent involvement of several organs and immunological abnormalities compared to those with normal levels. Furthermore, HMGB-1 and sRAGE levels correlated positively with modified Rodnan total skin thickness score and negatively with pulmonary function test. Conclusions: HMGB-1 and sRAGE expression in the sclerotic skin was more intense than normal skin. These results suggest that elevated serum HMGB-1 and sRAGE levels are associated with the disease severity and immunological abnormalities in SSc.

Original languageEnglish
Pages (from-to)180-189
Number of pages10
JournalJournal of Clinical Immunology
Volume29
Issue number2
DOIs
Publication statusPublished - 01-03-2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Clinical significance of serum HMGB-1 and sRAGE levels in systemic sclerosis: Association with disease severity'. Together they form a unique fingerprint.

  • Cite this

    Yoshizaki, A., Komura, K., Iwata, Y., Ogawa, F., Hara, T., Muroi, E., Takenaka, M., Shimizu, K., Hasegawa, M., Fujimoto, M., & Sato, S. (2009). Clinical significance of serum HMGB-1 and sRAGE levels in systemic sclerosis: Association with disease severity. Journal of Clinical Immunology, 29(2), 180-189. https://doi.org/10.1007/s10875-008-9252-x