Clinical usefulness of the PAXgeneTM bone marrow RNA system for stabilizing total RNA

Mayu Takeda, Tadao Funato, Masaki Ikemoto, Toru Nanmoku, Yoshimasa Urasaki, Yoshinori Iwatani

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The collection of clinical samples, such as bone marrow (BM) and peripheral blood, is an important procedure for the extraction of the cellular RNA. It is essential to preserve the extracted RNA during and after the collection of clinical samples to ensure the accurate analysis of gene expression. To date, the PAXgeneTM Blood RNA System has been proven useful for stabilizing RNA extracted from peripheral blood; however, a problem concerning the stability of the total RNA stored using the system has been identified. The PAXgeneTM Bone Marrow RNA System (BM system) is a newly developed system, and its clinical usefulness as a stabilizer for the cellular RNA in BM and peripheral blood was investigated with respect to the quality of RNA extracted using this system. A quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was carried out using total RNA extracted with the BM system, which showed that total RNA was more stable in the BM system than in the conventional system, indicating that the BM system can be applied to RT-PCR. The BM system enabled us to detect Wilms' tumor suppressor gene (WT1) more effectively than the conventional system. In conclusion, the BM system is clinically valuable for extracting and stabilizing total RNA of high quality.

Original languageEnglish
Pages (from-to)61-67
Number of pages7
JournalJournal of Clinical Laboratory Analysis
Volume29
Issue number1
DOIs
Publication statusPublished - 01-01-2015

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Hematology
  • Public Health, Environmental and Occupational Health
  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Biochemistry, medical
  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Clinical usefulness of the PAXgene<sup>TM</sup> bone marrow RNA system for stabilizing total RNA'. Together they form a unique fingerprint.

  • Cite this