Clonal Analysis of Multiple Point Mutations in the N‐ras Gene in Patients with Acute Myeloid Leukemia

Kazuaki Kubo, Tomoki Naoe, Hitoshi Kiyoi, Hisashi Fukutani, Yoshiro Kato, Takashi Oguri, Shunji Yamamori, Yoshiki Akatsuka, Yoshihisa Kodera, Ryuzo Ohno

Research output: Contribution to journalArticle

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Abstract

We have screened mutations of the N‐ras gene at codons 12, 13, and 61 in leukemia cells obtained from 100 patients with acute myeloid leukemia (AMD, and found mutated N‐ras alleles in 9 patients. We further analyzed the polyclonality of multiple N‐ras gene mutations in 4 AML patients. One patient, who had the monoclonal karyotype, t(11;17), had two types of double missense mutations at codons 13 and 61 in the same allele. Each of the remaining three patients, one of whom had t(15;17) with a monoclonal rearrangement of the retinoic acid receptor alpha and PML genes, carried two missense mutations in a relatively small population of leukemia cells. We have demonstrated that multiple clonality of the N‐ras gene is occasionally observed in leukemia with a monoclonal karyotype. These findings indicate that the N‐ras mutations may not always be characterized simply by an accumulative process and that the activated N‐ras gene alone is not sufficient to cause leukemia.

Original languageEnglish
Pages (from-to)379-387
Number of pages9
JournalJapanese Journal of Cancer Research
Volume84
Issue number4
DOIs
Publication statusPublished - 01-01-1993
Externally publishedYes

Fingerprint

Point Mutation
Acute Myeloid Leukemia
Leukemia
Genes
Missense Mutation
Karyotype
Codon
Mutation
Alleles
Population

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Kubo, Kazuaki ; Naoe, Tomoki ; Kiyoi, Hitoshi ; Fukutani, Hisashi ; Kato, Yoshiro ; Oguri, Takashi ; Yamamori, Shunji ; Akatsuka, Yoshiki ; Kodera, Yoshihisa ; Ohno, Ryuzo. / Clonal Analysis of Multiple Point Mutations in the N‐ras Gene in Patients with Acute Myeloid Leukemia. In: Japanese Journal of Cancer Research. 1993 ; Vol. 84, No. 4. pp. 379-387.
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Kubo, K, Naoe, T, Kiyoi, H, Fukutani, H, Kato, Y, Oguri, T, Yamamori, S, Akatsuka, Y, Kodera, Y & Ohno, R 1993, 'Clonal Analysis of Multiple Point Mutations in the N‐ras Gene in Patients with Acute Myeloid Leukemia', Japanese Journal of Cancer Research, vol. 84, no. 4, pp. 379-387. https://doi.org/10.1111/j.1349-7006.1993.tb00147.x

Clonal Analysis of Multiple Point Mutations in the N‐ras Gene in Patients with Acute Myeloid Leukemia. / Kubo, Kazuaki; Naoe, Tomoki; Kiyoi, Hitoshi; Fukutani, Hisashi; Kato, Yoshiro; Oguri, Takashi; Yamamori, Shunji; Akatsuka, Yoshiki; Kodera, Yoshihisa; Ohno, Ryuzo.

In: Japanese Journal of Cancer Research, Vol. 84, No. 4, 01.01.1993, p. 379-387.

Research output: Contribution to journalArticle

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AU - Kubo, Kazuaki

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AU - Kiyoi, Hitoshi

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AU - Oguri, Takashi

AU - Yamamori, Shunji

AU - Akatsuka, Yoshiki

AU - Kodera, Yoshihisa

AU - Ohno, Ryuzo

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N2 - We have screened mutations of the N‐ras gene at codons 12, 13, and 61 in leukemia cells obtained from 100 patients with acute myeloid leukemia (AMD, and found mutated N‐ras alleles in 9 patients. We further analyzed the polyclonality of multiple N‐ras gene mutations in 4 AML patients. One patient, who had the monoclonal karyotype, t(11;17), had two types of double missense mutations at codons 13 and 61 in the same allele. Each of the remaining three patients, one of whom had t(15;17) with a monoclonal rearrangement of the retinoic acid receptor alpha and PML genes, carried two missense mutations in a relatively small population of leukemia cells. We have demonstrated that multiple clonality of the N‐ras gene is occasionally observed in leukemia with a monoclonal karyotype. These findings indicate that the N‐ras mutations may not always be characterized simply by an accumulative process and that the activated N‐ras gene alone is not sufficient to cause leukemia.

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