Clonal growth of carp (Cyprinus carpio) T cells in vitro: Long-term proliferation of Th2-like cells

Takuya Yamaguchi, Fumihiko Katakura, Kazue Someya, J・m Dijkstra, Tadaaki Moritomo, Teruyuki Nakanishi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Carp kidney leukocytes co-cultured with a supporting cell layer resulted in proliferation of polyclonal CD4+ αβT cells as described previously. These bulk-cultured T cells expressed transcripts for both T helper 1 cells (Th1) master regulator (T-bet) and T helper 2 cells (Th2) master regulator (GATA-3). To identify the Th subsets in bulk-cultured T cells, single cells were picked up from the bulk culture, proliferated, and characterized. The majority of the clones displayed characteristics consistent with CD4+ αβT cell identity. These clones expressed both TCRα and TCRβ, but could not produce a TCRγδ heterodimer since they typically only expressed either TCRγ or TCRδ. These clones also expressed the TCR co-receptor genes CD4-1 or CD4-2, whereas they did not express CD8α or CD8β. In addition, GATA-3 was expressed whereas T-bet was not. Among these clones, one clone (KoThL5) continued to proliferate on the supporting cells and was successively transferred for more than 10 months and 90-100 passages. To characterize the KoThL5 cells by their cytokine production profile, they were stimulated with PHA and investigated by real-time RT-PCR. mRNA expression of Th2-related cytokine (IL-4/13B) was only enhanced in KoThL5 cells whereas both Th1-related cytokine (IFNγ) and Th2-related cytokines (IL-4/13A andIL-4/13B) were significantly enhanced in bulk-cultured T cells. Taken together, KoThL5 cells share some features with mammalian Th2 cells.This is the first study to describe in vitro cultures of teleost cell with Th2-like features. The KoThL5 cell line has considerable potential for addressing questions concerning the properties of teleost Th2 cells.

Original languageEnglish
Pages (from-to)433-442
Number of pages10
JournalFish and Shellfish Immunology
Volume34
Issue number2
DOIs
Publication statusPublished - 01-01-2013

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clonal growth
T-cells
Cyprinus carpio
carp
clone
T-lymphocytes
Cytokines
teleost
Interleukin-4
cells
Cells
clones
cytokines
cultured cells
Genes
Messenger RNA
interleukin-4
gene

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Cite this

Yamaguchi, Takuya ; Katakura, Fumihiko ; Someya, Kazue ; Dijkstra, J・m ; Moritomo, Tadaaki ; Nakanishi, Teruyuki. / Clonal growth of carp (Cyprinus carpio) T cells in vitro : Long-term proliferation of Th2-like cells. In: Fish and Shellfish Immunology. 2013 ; Vol. 34, No. 2. pp. 433-442.
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Clonal growth of carp (Cyprinus carpio) T cells in vitro : Long-term proliferation of Th2-like cells. / Yamaguchi, Takuya; Katakura, Fumihiko; Someya, Kazue; Dijkstra, J・m; Moritomo, Tadaaki; Nakanishi, Teruyuki.

In: Fish and Shellfish Immunology, Vol. 34, No. 2, 01.01.2013, p. 433-442.

Research output: Contribution to journalArticle

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AU - Yamaguchi, Takuya

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