TY - JOUR
T1 - Cloning and characterization of a human brain Na+-independent transporter for small neutral amino acids that transports D-serine with high affinity
AU - Nakauchi, Jun
AU - Matsuo, Hirotaka
AU - Kim, Do Kyung
AU - Goto, Akiteru
AU - Chairoungdua, Arthit
AU - Cha, Seok Ho
AU - Inatomi, Jun
AU - Shiokawa, Yoshiaki
AU - Yamaguchi, Kazuyoshi
AU - Saito, Isamu
AU - Endou, Hitoshi
AU - Kanai, Yoshikatsu
PY - 2000/6/30
Y1 - 2000/6/30
N2 - We isolated a cDNA for the human homologue of system asc transporter Asc-1 from human brain. The encoded protein designated as hAsc-1 (human Asc-1) exhibited 91 % sequence identity to mouse Asc-1. Consistent with mouse Asc-1, hAsc-1 required 4F2 heavy chain for its functional expression in Xenopus oocytes. hAsc-1 exhibited the properties of amino acid transport system asc which transports small neutral amino acids in a Na+-independent manner. hAsc-1 transported D-serine at high affinity with a K(m) value of 22.8 μM. In brain, 2.0 kb mRNA was highly expressed. hAsc-1 gene was mapped to human chromosome 19, region q12-q13.1. Because of the high-affinity transport with the K(m) value close to the physiological concentration of D-serine, together with the high levels of expression in brain, hAsc-1 is proposed to play significant roles in the D-serine mobilization in brain. Copyright (C) 2000 Elsevier Science Ireland Ltd.
AB - We isolated a cDNA for the human homologue of system asc transporter Asc-1 from human brain. The encoded protein designated as hAsc-1 (human Asc-1) exhibited 91 % sequence identity to mouse Asc-1. Consistent with mouse Asc-1, hAsc-1 required 4F2 heavy chain for its functional expression in Xenopus oocytes. hAsc-1 exhibited the properties of amino acid transport system asc which transports small neutral amino acids in a Na+-independent manner. hAsc-1 transported D-serine at high affinity with a K(m) value of 22.8 μM. In brain, 2.0 kb mRNA was highly expressed. hAsc-1 gene was mapped to human chromosome 19, region q12-q13.1. Because of the high-affinity transport with the K(m) value close to the physiological concentration of D-serine, together with the high levels of expression in brain, hAsc-1 is proposed to play significant roles in the D-serine mobilization in brain. Copyright (C) 2000 Elsevier Science Ireland Ltd.
KW - 4F2 Heavy chain
KW - Amino acid transporter
KW - D-Serine
KW - Human
KW - Human chromosome 19
KW - N-Methyl-D-aspartate receptor
KW - System asc
UR - https://www.scopus.com/pages/publications/0034733978
UR - https://www.scopus.com/pages/publications/0034733978#tab=citedBy
U2 - 10.1016/S0304-3940(00)01169-1
DO - 10.1016/S0304-3940(00)01169-1
M3 - Article
C2 - 10863037
AN - SCOPUS:0034733978
SN - 0304-3940
VL - 287
SP - 231
EP - 235
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 3
ER -