Cloning and characterization of a transmembrane serine kinase that acts as an activin type I receptor

K. Tsuchida, L. S. Mathews, W. W. Vale

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Activin type II receptors are transmembrane protein-serine/threonine kinases. By using a reverse-transcription PCR assay to screen for protein kinase sequences, we isolated a cDNA clone, activin X1 receptor, from rat brain that encodes a 55-kDa transmembrane protein-serine kinase which is structurally related to other receptors in this kinase subfamily. The predicted protein consists of 509 amino acids, and the kinase domain shows 40% and 37% identity to the activin and transforming growth factor β type II receptors, respectively. No activin-binding was observed when activin X1 receptor was expressed alone in COS-M6 cells; however, coexpression with type II activin receptors gave rise to a 68-kDa affinity-labeled complex in addition to the 85-kDa type II receptor complex. The size of this cross- linked band is consistent with the size of the type I activin receptor; furthermore, activin X1 receptor associated with type II receptors, as judged by coimmunoprecipitation with type II receptor antibodies. These data suggest that activin X1 receptor can serve as an activin type I receptor and that the diverse biological effects of activins may be mediated by a complex formed by the interaction of two transmembrane protein-serine kinases.

Original languageEnglish
Pages (from-to)11242-11246
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number23
DOIs
Publication statusPublished - 13-12-1993

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Type I Activin Receptors
Activin Receptors
Protein-Serine-Threonine Kinases
Activins
Organism Cloning
Type II Activin Receptors
Phosphotransferases
Growth Factor Receptors
COS Cells
Transforming Growth Factors
Protein Kinases
Reverse Transcription
Complementary DNA
Clone Cells
Amino Acids
Polymerase Chain Reaction
Antibodies
Brain
Proteins

All Science Journal Classification (ASJC) codes

  • General

Cite this

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Cloning and characterization of a transmembrane serine kinase that acts as an activin type I receptor. / Tsuchida, K.; Mathews, L. S.; Vale, W. W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 23, 13.12.1993, p. 11242-11246.

Research output: Contribution to journalArticle

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