Cloning and characterization of a transmembrane serine kinase that acts as an activin type I receptor

Kunihiro Tsuchida, Lawrence S. Mathews, Wylie W. Vale

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126 Citations (Scopus)


Activin type II receptors are transmembrane protein-serine/threonine kinases. By using a reverse-transcription PCR assay to screen for protein kinase sequences, we isolated a cDNA clone, activin X1 receptor, from rat brain that encodes a 55-kDa transmembrane protein-serine kinase which is structurally related to other receptors in this kinase subfamily. The predicted protein consists of 509 amino acids, and the kinase domain shows 40% and 37% identity to the activin and transforming growth factor β type II receptors, respectively. No activin-binding was observed when activin X1 receptor was expressed alone in COS-M6 cells; however, coexpression with type II activin receptors gave rise to a 68-kDa affinity-labeled complex in addition to the 85-kDa type II receptor complex. The size of this cross-linked band is consistent with the size of the type I activin receptor; furthermore, activin X1 receptor associated with type II receptors, as judged by coimmunoprecipitation with type II receptor antibodies. These data suggest that activin X1 receptor can serve as an activin type I receptor and that the diverse biological effects of activins may be mediated by a complex formed by the interaction of two transmembrane protein-serine kinases.

Original languageEnglish
Pages (from-to)11242-11246
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number23
Publication statusPublished - 01-12-1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General


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