TY - JOUR
T1 - Cloning and characterization of NE-dlg
T2 - A novel human homolog of the Drosophila discs large (dlg) tumor suppressor protein interacts with the APC protein
AU - Makino, Keishi
AU - Kuwahara, Hiroaki
AU - Masuko, Norio
AU - Nishiyama, Yasuyuki
AU - Morisaki, Tetsurou
AU - Sasaki, Ji Ichiro
AU - Nakao, Mitsuyoshi
AU - Kuwano, Akira
AU - Nakata, Motomi
AU - Ushio, Yukitaka
AU - Saya, Hideyuki
N1 - Funding Information:
We thank Drs K Tanaka and Y Takai, Department of Molecular Biology and Biochemistry, Osaka University Medical School, for technical advice revealed to yeast two-hybrid screening; Dr Q Hu for providing the pCGN expression vector; Dr H Maruta, Ludwig Institute for Cancer Research, for providing the pGEX-2TH bacterial expression vector; K Uriuda for secretarial assistance; and Dr J Moon for editing the manuscript. This work was supported by a grant for Cancer Research from the Ministry of Education, Science and Culture of Japan (HS). Genbank accession No. U49089.
PY - 1997
Y1 - 1997
N2 - We have cloned a cDNA for a novel human homolog of the Drosophila discs large (dlg) tumor suppressor protein, termed NE-dlg (neuronal and endocrine dlg). Northern blot analysis revealed that the gene is highly expressed in neuronal and endocrine tissues. Fluorescence in situ hybridization (FISH) and radiation hybrid mapping studies localized the NE-dlg gene to chromosome Xq13. We also found that the NE-dlg gene encoded a 100 kDa protein. Immunolocalization studies using an NE-dlg antibody showed that the protein tended to be expressed in non-proliferating cells, such as neurons, cells in Langerhans islets of the pancreas, myocytes of the heart muscles, and the prickle and functional layer cells of the esophageal epithelium. Proliferative cells, including various cultured cancer cell lines and basal cells in the esophageal epithelium, showed little expression of the NE-dlg protein. In addition, yeast two-hybrid screening and in vitro binding assays revealed that the NE-dlg interacted with the carboxyl-terminal region of the APC tumor suppressor protein. These data suggest that NE-dlg negatively regulates cell proliferation through its interaction with the APC protein.
AB - We have cloned a cDNA for a novel human homolog of the Drosophila discs large (dlg) tumor suppressor protein, termed NE-dlg (neuronal and endocrine dlg). Northern blot analysis revealed that the gene is highly expressed in neuronal and endocrine tissues. Fluorescence in situ hybridization (FISH) and radiation hybrid mapping studies localized the NE-dlg gene to chromosome Xq13. We also found that the NE-dlg gene encoded a 100 kDa protein. Immunolocalization studies using an NE-dlg antibody showed that the protein tended to be expressed in non-proliferating cells, such as neurons, cells in Langerhans islets of the pancreas, myocytes of the heart muscles, and the prickle and functional layer cells of the esophageal epithelium. Proliferative cells, including various cultured cancer cell lines and basal cells in the esophageal epithelium, showed little expression of the NE-dlg protein. In addition, yeast two-hybrid screening and in vitro binding assays revealed that the NE-dlg interacted with the carboxyl-terminal region of the APC tumor suppressor protein. These data suggest that NE-dlg negatively regulates cell proliferation through its interaction with the APC protein.
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U2 - 10.1038/sj.onc.1201087
DO - 10.1038/sj.onc.1201087
M3 - Article
C2 - 9188857
AN - SCOPUS:8544258802
SN - 0950-9232
VL - 14
SP - 2425
EP - 2433
JO - Oncogene
JF - Oncogene
IS - 20
ER -