Cloning and sequencing of a novel human gene that encodes a putative target protein of Nesh-SH3

S. Matsuda, C. Iriyama, S. Yokozaki, Y. Ichigotani, N. Shirafuji, K. Yamaki, T. Hayakawa, M. Hamaguchi

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

By using a conventional two-hybrid technique with an Src homology 3 (SH3) domain of Nesh as the bait protein, a novel full-length cDNA was isolated and sequenced from a human placenta cDNA library. This cDNA consists of 3023 bp and has a predicted open reading frame that encodes 486 amino acids. It possesses an SH3 binding motif, a nuclear targeting sequence, and no catalytic domain. Overall, it has no similarity to known molecules involved in a signaling cascade. Polymerase Chair reaction-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels localized the gene on human chromosome 3q12 near the marker D3S1271.

Original languageEnglish
Pages (from-to)483-486
Number of pages4
JournalJournal of Human Genetics
Volume46
Issue number8
DOIs
Publication statusPublished - 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

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