Cloning and sequencing of a novel human gene which encodes a putative hydroxylase

C. Iriyama, S. Matsuda, R. Katsumata, M. Hamaguchi

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Using a conventional two-hybrid technique with MAWD as bait protein, a novel full-length cDNA was isolated and sequenced from a human liver cDNA library. This cDNA consists of 2575 base pairs and has a predicted open reading frame encoding 255 amino acids. Overall, it is similar to the catalytic enzyme PHZF, catalyzing the hydroxylation of phenazine-1-carboxylic acid to 2-hydroxy-phenazine-1-carboxylic acid. Polymerase chain reaction-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels placed the gene to human chromosome 10q21.1 near the marker D10S210.

Original languageEnglish
Pages (from-to)289-292
Number of pages4
JournalJournal of Human Genetics
Issue number5
Publication statusPublished - 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)


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