Cloning and sequencing of cDNA encoding mouse GTP cyclohydrolase 1

Takahide Nomura, Hiroshi Ichinose, Chiho Sumi-Ichinose, Hiroko Nomura, Yasumichi Hagino, Keisuke Fujita, Toshiharu Nagatsu

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

A full-length cDNA clone for GTP cyclohydrolase I (EC 3.5.4.16) was isolated from a mouse brain cDNA library by plaque hybridization. The nucleotide sequence determination revealed that the length of the cDNA insert was 994 base pairs. The coding region encoded a protein of 241 amino acid residues with a calculated molecular mass of 27,014 daltons. The deduced amino acid sequence of mouse GTP cyclohydrolase I was found to be highly homologous to rat (96%) and human type 1(89%) enzymes.

Original languageEnglish
Pages (from-to)523-527
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume191
Issue number2
DOIs
Publication statusPublished - 15-03-1993

Fingerprint

GTP Cyclohydrolase
Cloning
Organism Cloning
Complementary DNA
Amino Acids
Molecular mass
Gene Library
Base Pairing
Sequence Analysis
Rats
Amino Acid Sequence
Brain
Nucleotides
Clone Cells
Enzymes
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Nomura, Takahide ; Ichinose, Hiroshi ; Sumi-Ichinose, Chiho ; Nomura, Hiroko ; Hagino, Yasumichi ; Fujita, Keisuke ; Nagatsu, Toshiharu. / Cloning and sequencing of cDNA encoding mouse GTP cyclohydrolase 1. In: Biochemical and Biophysical Research Communications. 1993 ; Vol. 191, No. 2. pp. 523-527.
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Cloning and sequencing of cDNA encoding mouse GTP cyclohydrolase 1. / Nomura, Takahide; Ichinose, Hiroshi; Sumi-Ichinose, Chiho; Nomura, Hiroko; Hagino, Yasumichi; Fujita, Keisuke; Nagatsu, Toshiharu.

In: Biochemical and Biophysical Research Communications, Vol. 191, No. 2, 15.03.1993, p. 523-527.

Research output: Contribution to journalArticle

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