Cloning of a cDNA Encoding a 190-kDa Insulin Receptor Substrate-1 (IRSS-1)-like Protein of Simian COS Cells

Lihong H. Wang, Hideki Hayashi, Yasumasa Mitani, Kazuo Ishii, Tetsuo Ohnishi, Yasuharu Niwa, Hiroshi Kido, Yousuke Ebina

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3 Citations (Scopus)

Abstract

Major insulin signals such as stimulation of glucose uptake and DNA synthesis and modification of hexose metabolism are mediated by the tyrosine-phosphorylated insulin receptor substrate-1 (IRS-1; pp 180) in many species of cells. We cloned cDNA encoding a 190-kDa IRS-1-like protein (pp190) in simian COS cells and which is slightly larger than IRS-1 (pp180) of human, rat, and mouse cells. The deduced amino acid sequence of COS pp190 consisted of 1251 amino acids and was 96.4%, 87.9% and 88.7% identical to human, mouse and rat IRS-1. The COS pp190 bound to SH2 (src-homology 2) domains of p85, Grb2/Ash, and SH-PTP2, as did IRS-1. In IRS-1-knockout mice, insulin signals are thought to be mediated by IRS-2 (pp190), which is an alternative signaling molecule and is slightly larger than IRS-1. However, the COS pp190 may be a simian homologue of IRS-1, but not of IRS-2. The results of Southern blotting suggested the possibility that Chinese hamster ovary (CHO) cells have not only the IRS-I gene but also a gene related to the COS pp190.

Original languageEnglish
Pages (from-to)321-328
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume216
Issue number1
DOIs
Publication statusPublished - 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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