Close Colocalization of CD4 and a Serine Esterase Tryptase TL2 on the Cell Surface of Human Monocytoid and CD4+ Lymphoid Cells

M. Inoue, T. Hoshino, T. Fukuma, Y. Niwa, H. Kido

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Tryptase TL2, a serine esterase in the membrane of human monocytoid and CD4+ lymphoid cells, specifically binds to the V3 domain of HIV-1 gp120. Here we report that monoclonal antibodies against CD4 that recognize the epitope interacting with gp120 specifically blocked the immunostaining of cell-surface tryptase TL2, although the antibody does not crossreact with tryptase TL2. Down-regulation of cell-surface CD4 induced by HIV-1 Nef prevented this blocking effect. These data suggest that CD4 is closely co-localized with tryptase TL2 on the cell surface and that regulation of the expression of tryptase TL2 is not associated with that of CD4.

Original languageEnglish
Pages (from-to)1390-1395
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume201
Issue number3
DOIs
Publication statusPublished - 30-06-1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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