Closely linked polymorphic markers for determining the autosomal dominant allele (Cy) in rat polycystic kidney disease

Shizuko Nagao, Toshikazu Ushijima, Masao Kasahara, Tamio Yamaguchi, Masatomo Kusaka, Jun Matsuda, Minako Nagao, Hisahide Takahashi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The Han:SPRD strain is an SD-background strain known to be a model of polycystic kidney disease (PKD) expressed through an autosomal dominant gene (Cy). However, different genotypes of this strain cannot be identified in the neonatal period. First, to establish an accurate method of determining the genotypes (Cy/Cy, Cy/+, +/+) which cause different disease progressions, we used polymorphic markers on rat chromosome 5. PCR products of tissue DNA templated with D5Rat9 showed distinct patterns on electrophoresis indicating three genotypes. Second, to determine whether the same locus plays a major role in expressing PKD, we performed linkage analyses in a [BN x (BN x Han:SPRD)F1] backcross. Cy/Cy and Cy/+ also caused PKD in a BN background. In this backcross, we discovered that D5Rat11 is located closer to the Cy locus than D5Mgh10, which is regarded as one of the closest loci. We conclude that D5Rat9 and D5Rat11 are useful markers for determining the presence of the Cy allele, which is regarded as the gene responsible for PKD.

Original languageEnglish
Pages (from-to)227-235
Number of pages9
JournalBiochemical Genetics
Volume37
Issue number7-8
DOIs
Publication statusPublished - 22-11-1999

Fingerprint

Polycystic Kidney Diseases
Rats
allele
Alleles
alleles
genotype
rats
Genotype
loci
Dominant Genes
Chromosomes, Human, Pair 5
Genes
gene
dominant genes
disease course
electrophoresis
linkage (genetics)
Disease Progression
Electrophoresis
chromosome

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Nagao, Shizuko ; Ushijima, Toshikazu ; Kasahara, Masao ; Yamaguchi, Tamio ; Kusaka, Masatomo ; Matsuda, Jun ; Nagao, Minako ; Takahashi, Hisahide. / Closely linked polymorphic markers for determining the autosomal dominant allele (Cy) in rat polycystic kidney disease. In: Biochemical Genetics. 1999 ; Vol. 37, No. 7-8. pp. 227-235.
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Nagao, S, Ushijima, T, Kasahara, M, Yamaguchi, T, Kusaka, M, Matsuda, J, Nagao, M & Takahashi, H 1999, 'Closely linked polymorphic markers for determining the autosomal dominant allele (Cy) in rat polycystic kidney disease', Biochemical Genetics, vol. 37, no. 7-8, pp. 227-235. https://doi.org/10.1023/A:1018742807031

Closely linked polymorphic markers for determining the autosomal dominant allele (Cy) in rat polycystic kidney disease. / Nagao, Shizuko; Ushijima, Toshikazu; Kasahara, Masao; Yamaguchi, Tamio; Kusaka, Masatomo; Matsuda, Jun; Nagao, Minako; Takahashi, Hisahide.

In: Biochemical Genetics, Vol. 37, No. 7-8, 22.11.1999, p. 227-235.

Research output: Contribution to journalArticle

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AU - Nagao, Shizuko

AU - Ushijima, Toshikazu

AU - Kasahara, Masao

AU - Yamaguchi, Tamio

AU - Kusaka, Masatomo

AU - Matsuda, Jun

AU - Nagao, Minako

AU - Takahashi, Hisahide

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N2 - The Han:SPRD strain is an SD-background strain known to be a model of polycystic kidney disease (PKD) expressed through an autosomal dominant gene (Cy). However, different genotypes of this strain cannot be identified in the neonatal period. First, to establish an accurate method of determining the genotypes (Cy/Cy, Cy/+, +/+) which cause different disease progressions, we used polymorphic markers on rat chromosome 5. PCR products of tissue DNA templated with D5Rat9 showed distinct patterns on electrophoresis indicating three genotypes. Second, to determine whether the same locus plays a major role in expressing PKD, we performed linkage analyses in a [BN x (BN x Han:SPRD)F1] backcross. Cy/Cy and Cy/+ also caused PKD in a BN background. In this backcross, we discovered that D5Rat11 is located closer to the Cy locus than D5Mgh10, which is regarded as one of the closest loci. We conclude that D5Rat9 and D5Rat11 are useful markers for determining the presence of the Cy allele, which is regarded as the gene responsible for PKD.

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