TY - JOUR
T1 - Closely linked polymorphic markers for determining the autosomal dominant allele (Cy) in rat polycystic kidney disease
AU - Nagao, Shizuko
AU - Ushijima, Toshikazu
AU - Kasahara, Masao
AU - Yamaguchi, Tamio
AU - Kusaka, Masatomo
AU - Matsuda, Jun
AU - Nagao, Minako
AU - Takahashi, Hisahide
N1 - Funding Information:
We aregrateful to Drs. Benjamin D. Cowley, Jr., and Jared J. Grantham (University of Kansas Medical Center) for their kind gift of the Han:SPRD strain. WethankDr.TomomasaWatanabe(HokkaidoUniversit,yJapn)afroscineti® c advice. This study was supported in part by grants-in-aid from the Ministry of Education, Science and Culture of Japan and from Fujita Health University.
PY - 1999
Y1 - 1999
N2 - The Han:SPRD strain is an SD-background strain known to be a model of polycystic kidney disease (PKD) expressed through an autosomal dominant gene (Cy). However, different genotypes of this strain cannot be identified in the neonatal period. First, to establish an accurate method of determining the genotypes (Cy/Cy, Cy/+, +/+) which cause different disease progressions, we used polymorphic markers on rat chromosome 5. PCR products of tissue DNA templated with D5Rat9 showed distinct patterns on electrophoresis indicating three genotypes. Second, to determine whether the same locus plays a major role in expressing PKD, we performed linkage analyses in a [BN x (BN x Han:SPRD)F1] backcross. Cy/Cy and Cy/+ also caused PKD in a BN background. In this backcross, we discovered that D5Rat11 is located closer to the Cy locus than D5Mgh10, which is regarded as one of the closest loci. We conclude that D5Rat9 and D5Rat11 are useful markers for determining the presence of the Cy allele, which is regarded as the gene responsible for PKD.
AB - The Han:SPRD strain is an SD-background strain known to be a model of polycystic kidney disease (PKD) expressed through an autosomal dominant gene (Cy). However, different genotypes of this strain cannot be identified in the neonatal period. First, to establish an accurate method of determining the genotypes (Cy/Cy, Cy/+, +/+) which cause different disease progressions, we used polymorphic markers on rat chromosome 5. PCR products of tissue DNA templated with D5Rat9 showed distinct patterns on electrophoresis indicating three genotypes. Second, to determine whether the same locus plays a major role in expressing PKD, we performed linkage analyses in a [BN x (BN x Han:SPRD)F1] backcross. Cy/Cy and Cy/+ also caused PKD in a BN background. In this backcross, we discovered that D5Rat11 is located closer to the Cy locus than D5Mgh10, which is regarded as one of the closest loci. We conclude that D5Rat9 and D5Rat11 are useful markers for determining the presence of the Cy allele, which is regarded as the gene responsible for PKD.
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U2 - 10.1023/A:1018742807031
DO - 10.1023/A:1018742807031
M3 - Article
C2 - 10624513
AN - SCOPUS:0032708294
VL - 37
SP - 227
EP - 235
JO - Biochemical Genetics
JF - Biochemical Genetics
SN - 0006-2928
IS - 7-8
ER -