TY - JOUR
T1 - Co-Cr-Mo alloy particles induce tumor necrosis factor alpha production in MLO-Y4 osteocytes
T2 - A role for osteocytes in particle-induced inflammation
AU - Kanaji, Arihiko
AU - Caicedo, Marco S.
AU - Virdi, Amarjit S.
AU - Sumner, D. Rick
AU - Hallab, Nadim J.
AU - Sena, Kotaro
N1 - Funding Information:
The authors gratefully thank Dr. Lynda F. Bonewald, University of Missouri-Kansas City for providing the MLO-Y4 cells and David G. Karwo for his assistance. The project described was supported by Grant Number AR054171 from NIH/NIAMS and the Grainger Foundation.
PY - 2009/9
Y1 - 2009/9
N2 - Wear debris-induced osteolysis is purportedly the limiting problem affecting the long term results of joint arthroplasty. Pathogenic effects of wear debris in peri-implant cells such as macrophages, osteoblasts and osteoclasts have been well studied. In contrast, the effects of wear debris on osteocytes, which make up over 90% of all bone cells, remain unknown. We hypothesized that metal implant debris can induce the pro-inflammatory response in osteocytes. This study demonstrated the effects of cobalt-chromium-molybdenum alloy (Co-Cr-Mo) particles on a well-characterized MLO-Y4 osteocyte cell line. Co-Cr-Mo alloy particle treatment significantly (p < 0.05) up-regulated tumor necrosis factor alpha (TNFα) gene expression after 3 and 6 h and TNFα protein production after 24 h, but down-regulated interleukin-6 (IL-6) gene expression after 6 h. Co-Cr-Mo alloy particle treatment also induced osteocyte apoptosis after 24 h. This apoptotic effect was partially (40%) dependent on TNFα. Therefore, our results suggest that osteocytes play a role in particle-induced inflammation and bone resorption following total joint arthroplasty by inducing pro-inflammatory cytokines and inducing osteocyte apoptosis.
AB - Wear debris-induced osteolysis is purportedly the limiting problem affecting the long term results of joint arthroplasty. Pathogenic effects of wear debris in peri-implant cells such as macrophages, osteoblasts and osteoclasts have been well studied. In contrast, the effects of wear debris on osteocytes, which make up over 90% of all bone cells, remain unknown. We hypothesized that metal implant debris can induce the pro-inflammatory response in osteocytes. This study demonstrated the effects of cobalt-chromium-molybdenum alloy (Co-Cr-Mo) particles on a well-characterized MLO-Y4 osteocyte cell line. Co-Cr-Mo alloy particle treatment significantly (p < 0.05) up-regulated tumor necrosis factor alpha (TNFα) gene expression after 3 and 6 h and TNFα protein production after 24 h, but down-regulated interleukin-6 (IL-6) gene expression after 6 h. Co-Cr-Mo alloy particle treatment also induced osteocyte apoptosis after 24 h. This apoptotic effect was partially (40%) dependent on TNFα. Therefore, our results suggest that osteocytes play a role in particle-induced inflammation and bone resorption following total joint arthroplasty by inducing pro-inflammatory cytokines and inducing osteocyte apoptosis.
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U2 - 10.1016/j.bone.2009.05.020
DO - 10.1016/j.bone.2009.05.020
M3 - Article
C2 - 19497395
AN - SCOPUS:67949120307
SN - 8756-3282
VL - 45
SP - 528
EP - 533
JO - Bone
JF - Bone
IS - 3
ER -
