Coated glass slides TACAS are applicable to heat-assisted immunostaining and In situ hybridization at the electron microscopy level

Takahiro Matsui, Takanori Onouchi, Kazuya Shiogama, Yasuyoshi Mizutani, Ken Ichi Inada, Fuxun Yu, Daisuke Hayasaka, Koichi Morita, Hirohisa Ogawa, Fumihiko Mahara, Yutaka Tsutsumi

Research output: Contribution to journalComment/debate

4 Citations (Scopus)

Abstract

We performed pre-embedding electron microscopic study for visualizing the antigen and genome of severe fever with thrombocytopenia syndrome (SFTS) virus in the cytoplasm of macrophages of the human splenic red pulp, both requesting preheating treatment of sections. To pursue this, coated glass slides with unique characteristics are needed. Namely, during staining they must prevent detaching off sections, but after staining the sections must be transferred to epoxy resin. Aminopropyltriexoxysilane-coated glass slides, widely used for immunostaining, were resistant to transfer to epoxy resin. In contrast, coated glass slides designated as Thinlayer Advanced Cytology Assay System (TACAS) were suitable for this purpose. The technique is also applicable to the coated glass slide-requiring cytology practice, in which immunocytochemical evaluation is needed after cell transfer to another glass slide.

Original languageEnglish
Pages (from-to)153-157
Number of pages5
JournalActa Histochemica et Cytochemica
Volume48
Issue number5
DOIs
Publication statusPublished - 29-10-2015

Fingerprint

Cytology
Electron microscopy
In Situ Hybridization
Glass
Cell Biology
Assays
Electron Microscopy
Hot Temperature
Epoxy Resins
Staining and Labeling
Macrophages
Preheating
Viruses
Thrombocytopenia
Pulp
Cytoplasm
Fever
Genes
Genome
Electrons

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

Cite this

Matsui, Takahiro ; Onouchi, Takanori ; Shiogama, Kazuya ; Mizutani, Yasuyoshi ; Inada, Ken Ichi ; Yu, Fuxun ; Hayasaka, Daisuke ; Morita, Koichi ; Ogawa, Hirohisa ; Mahara, Fumihiko ; Tsutsumi, Yutaka. / Coated glass slides TACAS are applicable to heat-assisted immunostaining and In situ hybridization at the electron microscopy level. In: Acta Histochemica et Cytochemica. 2015 ; Vol. 48, No. 5. pp. 153-157.
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abstract = "We performed pre-embedding electron microscopic study for visualizing the antigen and genome of severe fever with thrombocytopenia syndrome (SFTS) virus in the cytoplasm of macrophages of the human splenic red pulp, both requesting preheating treatment of sections. To pursue this, coated glass slides with unique characteristics are needed. Namely, during staining they must prevent detaching off sections, but after staining the sections must be transferred to epoxy resin. Aminopropyltriexoxysilane-coated glass slides, widely used for immunostaining, were resistant to transfer to epoxy resin. In contrast, coated glass slides designated as Thinlayer Advanced Cytology Assay System (TACAS) were suitable for this purpose. The technique is also applicable to the coated glass slide-requiring cytology practice, in which immunocytochemical evaluation is needed after cell transfer to another glass slide.",
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Coated glass slides TACAS are applicable to heat-assisted immunostaining and In situ hybridization at the electron microscopy level. / Matsui, Takahiro; Onouchi, Takanori; Shiogama, Kazuya; Mizutani, Yasuyoshi; Inada, Ken Ichi; Yu, Fuxun; Hayasaka, Daisuke; Morita, Koichi; Ogawa, Hirohisa; Mahara, Fumihiko; Tsutsumi, Yutaka.

In: Acta Histochemica et Cytochemica, Vol. 48, No. 5, 29.10.2015, p. 153-157.

Research output: Contribution to journalComment/debate

TY - JOUR

T1 - Coated glass slides TACAS are applicable to heat-assisted immunostaining and In situ hybridization at the electron microscopy level

AU - Matsui, Takahiro

AU - Onouchi, Takanori

AU - Shiogama, Kazuya

AU - Mizutani, Yasuyoshi

AU - Inada, Ken Ichi

AU - Yu, Fuxun

AU - Hayasaka, Daisuke

AU - Morita, Koichi

AU - Ogawa, Hirohisa

AU - Mahara, Fumihiko

AU - Tsutsumi, Yutaka

PY - 2015/10/29

Y1 - 2015/10/29

N2 - We performed pre-embedding electron microscopic study for visualizing the antigen and genome of severe fever with thrombocytopenia syndrome (SFTS) virus in the cytoplasm of macrophages of the human splenic red pulp, both requesting preheating treatment of sections. To pursue this, coated glass slides with unique characteristics are needed. Namely, during staining they must prevent detaching off sections, but after staining the sections must be transferred to epoxy resin. Aminopropyltriexoxysilane-coated glass slides, widely used for immunostaining, were resistant to transfer to epoxy resin. In contrast, coated glass slides designated as Thinlayer Advanced Cytology Assay System (TACAS) were suitable for this purpose. The technique is also applicable to the coated glass slide-requiring cytology practice, in which immunocytochemical evaluation is needed after cell transfer to another glass slide.

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