TY - JOUR
T1 - Collagen immobilized PVA hydrogel-hydroxyapatite composites prepared by kneading methods as a material for peripheral cuff of artificial cornea
AU - Kobayashi, Hisatoshi
AU - Kato, Masabumi
AU - Taguchi, Tetsushi
AU - Ikoma, Toshiyuki
AU - Miyashita, Hideyuki
AU - Shimmura, Shigeto
AU - Tsubota, Kazuo
AU - Tanaka, Junzo
N1 - Funding Information:
This study was financially supported in part by grant-in-Aid for Scientific Researches (1301B/15300176) and Leading Project (the contract No. (15–99 and) 15–396) and advanced and innovational research program in life sciences from the ministry of Education, Culture, Sports, Science and Technology(MEXT), the Japanese Government.
PY - 2004/12/1
Y1 - 2004/12/1
N2 - In order to achieve the firm fixation of the artificial cornea to host tissues, composites of collagen-immobilized poly(vinyl alcohol) hydrogel with hydroxyapatite were synthesized by a hydroxyapatite particles kneading method. The preparation method, characterization, and the results of corneal cell adhesion and proliferation on the composite material were studied. PVA-COL-HAp composites were successfully synthesized. A micro-porous structure of the PVA-COL-HAp could be introduced by hydrochloric acid treatment and the porosity could be controlled by the pH of the hydrochloric acid solution, the treatment time, and the crystallinity of the HAp particles, Chick embryonic keratocyto-like cells were well attached and proliferated on the PVA-COL-HAp composites. This material showed potential for keratoprosthesis application. Further study such as a long-term animal study is now required.
AB - In order to achieve the firm fixation of the artificial cornea to host tissues, composites of collagen-immobilized poly(vinyl alcohol) hydrogel with hydroxyapatite were synthesized by a hydroxyapatite particles kneading method. The preparation method, characterization, and the results of corneal cell adhesion and proliferation on the composite material were studied. PVA-COL-HAp composites were successfully synthesized. A micro-porous structure of the PVA-COL-HAp could be introduced by hydrochloric acid treatment and the porosity could be controlled by the pH of the hydrochloric acid solution, the treatment time, and the crystallinity of the HAp particles, Chick embryonic keratocyto-like cells were well attached and proliferated on the PVA-COL-HAp composites. This material showed potential for keratoprosthesis application. Further study such as a long-term animal study is now required.
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U2 - 10.1016/j.msec.2004.08.038
DO - 10.1016/j.msec.2004.08.038
M3 - Article
AN - SCOPUS:9444278385
SN - 0928-4931
VL - 24
SP - 729
EP - 735
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
IS - 6-8 SPEC. ISS.
ER -