Colorectal cancer cell-derived microvesicles containing microRNA-1246 promote angiogenesis by activating Smad 1/5/8 signaling elicited by PML down-regulation in endothelial cells

Nami Yamada, Nonoka Tsujimura, Minami Kumazaki, Haruka Shinohara, Kohei Taniguchi, Yoshihito Nakagawa, Tomoki Naoe, Yukihiro Akao

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Emerging studies on circulating microRNAs (miRNAs) or microvesicles (MVs) have shown the potential of them to be novel biomarkers and therapeutic targets for cancer. However, the biological roles of these miRNAs and MVs have not been validated yet. To determine the biological significance of MVs, we used human colorectal cancer cells as the MV donor and endothelial cells (HUVECs) as the MV recipient and demonstrated the transfer of colorectal cancer cell-derived MVs (CRC-MVs) to HUVECs and evaluated the roles of these MVs and their cargo in tumor angiogenesis. Consequently, the incubation of HUVECs with CRC-MVs promoted the proliferation, migration, and tube formation activities of these cells. Among the cargoes shuttled by the MVs, miR-1246 and TGF-β were considered to be responsible for the pro-angiogenic function of MVs by activating Smad 1/5/8 signaling in the HUVECs. These results suggest that colorectal cancer cells secreted MVs to contribute to tumor angiogenesis.

Original languageEnglish
Pages (from-to)1256-1272
Number of pages17
JournalBiochimica et Biophysica Acta - Gene Regulatory Mechanisms
Volume1839
Issue number11
DOIs
Publication statusPublished - 01-11-2014

Fingerprint

Endothelial cells
MicroRNAs
Colorectal Neoplasms
Down-Regulation
Endothelial Cells
Cells
Tumors
Neoplasms
Biomarkers

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Yamada, Nami ; Tsujimura, Nonoka ; Kumazaki, Minami ; Shinohara, Haruka ; Taniguchi, Kohei ; Nakagawa, Yoshihito ; Naoe, Tomoki ; Akao, Yukihiro. / Colorectal cancer cell-derived microvesicles containing microRNA-1246 promote angiogenesis by activating Smad 1/5/8 signaling elicited by PML down-regulation in endothelial cells. In: Biochimica et Biophysica Acta - Gene Regulatory Mechanisms. 2014 ; Vol. 1839, No. 11. pp. 1256-1272.
@article{b08c0c1c9cbb454ea617d0da3b36f646,
title = "Colorectal cancer cell-derived microvesicles containing microRNA-1246 promote angiogenesis by activating Smad 1/5/8 signaling elicited by PML down-regulation in endothelial cells",
abstract = "Emerging studies on circulating microRNAs (miRNAs) or microvesicles (MVs) have shown the potential of them to be novel biomarkers and therapeutic targets for cancer. However, the biological roles of these miRNAs and MVs have not been validated yet. To determine the biological significance of MVs, we used human colorectal cancer cells as the MV donor and endothelial cells (HUVECs) as the MV recipient and demonstrated the transfer of colorectal cancer cell-derived MVs (CRC-MVs) to HUVECs and evaluated the roles of these MVs and their cargo in tumor angiogenesis. Consequently, the incubation of HUVECs with CRC-MVs promoted the proliferation, migration, and tube formation activities of these cells. Among the cargoes shuttled by the MVs, miR-1246 and TGF-β were considered to be responsible for the pro-angiogenic function of MVs by activating Smad 1/5/8 signaling in the HUVECs. These results suggest that colorectal cancer cells secreted MVs to contribute to tumor angiogenesis.",
author = "Nami Yamada and Nonoka Tsujimura and Minami Kumazaki and Haruka Shinohara and Kohei Taniguchi and Yoshihito Nakagawa and Tomoki Naoe and Yukihiro Akao",
year = "2014",
month = "11",
day = "1",
doi = "10.1016/j.bbagrm.2014.09.002",
language = "English",
volume = "1839",
pages = "1256--1272",
journal = "Biochimica et Biophysica Acta - Gene Regulatory Mechanisms",
issn = "1874-9399",
publisher = "Elsevier",
number = "11",

}

Colorectal cancer cell-derived microvesicles containing microRNA-1246 promote angiogenesis by activating Smad 1/5/8 signaling elicited by PML down-regulation in endothelial cells. / Yamada, Nami; Tsujimura, Nonoka; Kumazaki, Minami; Shinohara, Haruka; Taniguchi, Kohei; Nakagawa, Yoshihito; Naoe, Tomoki; Akao, Yukihiro.

In: Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, Vol. 1839, No. 11, 01.11.2014, p. 1256-1272.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Colorectal cancer cell-derived microvesicles containing microRNA-1246 promote angiogenesis by activating Smad 1/5/8 signaling elicited by PML down-regulation in endothelial cells

AU - Yamada, Nami

AU - Tsujimura, Nonoka

AU - Kumazaki, Minami

AU - Shinohara, Haruka

AU - Taniguchi, Kohei

AU - Nakagawa, Yoshihito

AU - Naoe, Tomoki

AU - Akao, Yukihiro

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Emerging studies on circulating microRNAs (miRNAs) or microvesicles (MVs) have shown the potential of them to be novel biomarkers and therapeutic targets for cancer. However, the biological roles of these miRNAs and MVs have not been validated yet. To determine the biological significance of MVs, we used human colorectal cancer cells as the MV donor and endothelial cells (HUVECs) as the MV recipient and demonstrated the transfer of colorectal cancer cell-derived MVs (CRC-MVs) to HUVECs and evaluated the roles of these MVs and their cargo in tumor angiogenesis. Consequently, the incubation of HUVECs with CRC-MVs promoted the proliferation, migration, and tube formation activities of these cells. Among the cargoes shuttled by the MVs, miR-1246 and TGF-β were considered to be responsible for the pro-angiogenic function of MVs by activating Smad 1/5/8 signaling in the HUVECs. These results suggest that colorectal cancer cells secreted MVs to contribute to tumor angiogenesis.

AB - Emerging studies on circulating microRNAs (miRNAs) or microvesicles (MVs) have shown the potential of them to be novel biomarkers and therapeutic targets for cancer. However, the biological roles of these miRNAs and MVs have not been validated yet. To determine the biological significance of MVs, we used human colorectal cancer cells as the MV donor and endothelial cells (HUVECs) as the MV recipient and demonstrated the transfer of colorectal cancer cell-derived MVs (CRC-MVs) to HUVECs and evaluated the roles of these MVs and their cargo in tumor angiogenesis. Consequently, the incubation of HUVECs with CRC-MVs promoted the proliferation, migration, and tube formation activities of these cells. Among the cargoes shuttled by the MVs, miR-1246 and TGF-β were considered to be responsible for the pro-angiogenic function of MVs by activating Smad 1/5/8 signaling in the HUVECs. These results suggest that colorectal cancer cells secreted MVs to contribute to tumor angiogenesis.

UR - http://www.scopus.com/inward/record.url?scp=84907732356&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907732356&partnerID=8YFLogxK

U2 - 10.1016/j.bbagrm.2014.09.002

DO - 10.1016/j.bbagrm.2014.09.002

M3 - Article

C2 - 25218966

AN - SCOPUS:84907732356

VL - 1839

SP - 1256

EP - 1272

JO - Biochimica et Biophysica Acta - Gene Regulatory Mechanisms

JF - Biochimica et Biophysica Acta - Gene Regulatory Mechanisms

SN - 1874-9399

IS - 11

ER -