TY - JOUR
T1 - Combination of neonatal PolyI
T2 - C and adolescent phencyclidine treatments is required to induce behavioral abnormalities with overexpression of GLAST in adult mice
AU - Hida, Hirotake
AU - Mouri, Akihiro
AU - Ando, Yu
AU - Mori, Kentaro
AU - Mamiya, Takayoshi
AU - Iwamoto, Kunihiro
AU - Ozaki, Norio
AU - Yamada, Kiyofumi
AU - Nabeshima, Toshitaka
AU - Noda, Yukihiro
N1 - Funding Information:
This study was supported by the ‘Academic Frontier’ Project for Private Universities (2007–2011), Grants-in-Aid for Scientific Research A ( 22248033 ), Scientific Research B ( 22659213 ) and Scientific Research C ( 24590219 ) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan ; by Research on Risk of Chemical Substances (2008–2010) , Health and Labor Science Research Grants supported by the Ministry of Health, Labor and Welfare (MHLW) ; by a research grant from the Research Institute of Meijo University ; by the joint research project under the Japan-Korea Basic Scientific Cooperation Program ; by a Smoking Research Foundation Grant for Biomedical Research (SRF) ; by the joint research project under the Japan-Korea basic scientific cooperation program (2010–2012); and by a Grant-in-Aid for Young Scientists (B) (23791325) from Japan Society for the Promotion of Science (JSPS) .
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Cumulative incidences of multiple risk factors are related to pathology of psychiatric disorders. The present study was designed to examine combinative effects of a neonatal immune challenge with adolescent abused substance treatment on the psychological behaviors and molecular expressions in the adult. C57BL/6J mice were neonatally treated, with polyriboinosinic-polyribocytidylic acid (PolyI:C: 5. mg/kg) during postnatal days (PD) 2-6, then with phencyclidine (PCP: 10. mg/kg) during adolescence (PD35-41). Locomotor activity was analyzed to evaluate sensitivity to PCP on PD35 and PD41. Emotional and cognitive tests were carried out on PD42-48. Neonatal PolyI:C treatment markedly enhanced sensitivity to PCP- and methamphetamine-induced hyperactivity in the adolescent. Mice treated with both neonatal PolyI:C and adolescent PCP (PolyI:C/PCP) showed social deficit and object recognition memory impairment. The expression of glutamate/aspartate transporter (GLAST) in the prefrontal cortex (PFC) was significantly increased in the (PolyI:C/PCP)-treated mice. Infusion of glutamate transporter inhibitor (DL-TBOA: 1. nmol/bilaterally) into the PFC reversed the object recognition impairment in the (PolyI:C/PCP)-treated mice. These results indicate that the combined treatment of neonatal PolyI:C with adolescent PCP leads to behavioral abnormalities, which were associated with increase of GLAST expression in the adult PFC.
AB - Cumulative incidences of multiple risk factors are related to pathology of psychiatric disorders. The present study was designed to examine combinative effects of a neonatal immune challenge with adolescent abused substance treatment on the psychological behaviors and molecular expressions in the adult. C57BL/6J mice were neonatally treated, with polyriboinosinic-polyribocytidylic acid (PolyI:C: 5. mg/kg) during postnatal days (PD) 2-6, then with phencyclidine (PCP: 10. mg/kg) during adolescence (PD35-41). Locomotor activity was analyzed to evaluate sensitivity to PCP on PD35 and PD41. Emotional and cognitive tests were carried out on PD42-48. Neonatal PolyI:C treatment markedly enhanced sensitivity to PCP- and methamphetamine-induced hyperactivity in the adolescent. Mice treated with both neonatal PolyI:C and adolescent PCP (PolyI:C/PCP) showed social deficit and object recognition memory impairment. The expression of glutamate/aspartate transporter (GLAST) in the prefrontal cortex (PFC) was significantly increased in the (PolyI:C/PCP)-treated mice. Infusion of glutamate transporter inhibitor (DL-TBOA: 1. nmol/bilaterally) into the PFC reversed the object recognition impairment in the (PolyI:C/PCP)-treated mice. These results indicate that the combined treatment of neonatal PolyI:C with adolescent PCP leads to behavioral abnormalities, which were associated with increase of GLAST expression in the adult PFC.
UR - http://www.scopus.com/inward/record.url?scp=84886853802&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84886853802&partnerID=8YFLogxK
U2 - 10.1016/j.bbr.2013.09.026
DO - 10.1016/j.bbr.2013.09.026
M3 - Article
C2 - 24060653
AN - SCOPUS:84886853802
SN - 0166-4328
VL - 258
SP - 34
EP - 42
JO - Behavioural Brain Research
JF - Behavioural Brain Research
ER -