Comparison of specific serological assays for diagnosing human herpesvirus 6 infection after liver transplantation

T. Yoshikawa, J. B. Black, M. Ihira, K. Suzuki, S. Suga, K. Iida, Y. Saito, K. Asonuma, K. Tanaka, Y. Asano

Research output: Contribution to journalArticle

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Abstract

Cross-reactivity between human herpesvirus 6 (HHV-6) and human herpesvirus 7 (HHV-7) antibodies and the reliability of specific serological assays were analyzed for 12 patients with concurrent HHV-6 and HHV-7 antibody responses after transplantation with a liver from a living relative by using an immunofluorescence assay (IFA). A neutralizing antibody titer assay (NT) and an immunoblot assay (IB) designed to detect immunoglobulin M (IgM) antibody to the HHV-6 immunodominant 101-kDa protein were compared in the diagnosis of an active HHV-6 infection. A total of 9 of 12 patients demonstrated concurrent HHV-6 and HHV-7 antibody responses, including increased IgG titers and/or the presence of IgM by IFA, and were thus analyzed for cross-reactive antibody to heterologous virus. The average percentages of residual antibody to HHV-6 and HHV-7 after absorption with HHV-6 antigen were 32.6% (range, 6 to 50%) and 55.6% (range, 35 to 100%), respectively. All 12 patients were subsequently analyzed for HHV-6 antibody by using IB and NT. IB detected IgM antibody to the 101-kDa protein in 75% (9 of 12) of the recipients. A significant rise in the NT antibody titer was detected in the same nine samples. However, HHV-6 DNA was detected by PCR in only five of nine plasma samples collected from recipients with a specific serologic response against HHV-6.

Original languageEnglish
Pages (from-to)170-173
Number of pages4
JournalClinical and Diagnostic Laboratory Immunology
Volume8
Issue number1
DOIs
Publication statusPublished - 31-01-2001

Fingerprint

Human Herpesvirus 6
Herpesviridae Infections
Liver
Liver Transplantation
Assays
Human Herpesvirus 7
Antibodies
Immunoglobulin M
Antibody Formation
Fluorescent Antibody Technique
Heterophile Antibodies
Neutralizing Antibodies
Viruses
Proteins
Immunoglobulin G
Transplantation
Plasmas
Antigens

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

Cite this

Yoshikawa, T. ; Black, J. B. ; Ihira, M. ; Suzuki, K. ; Suga, S. ; Iida, K. ; Saito, Y. ; Asonuma, K. ; Tanaka, K. ; Asano, Y. / Comparison of specific serological assays for diagnosing human herpesvirus 6 infection after liver transplantation. In: Clinical and Diagnostic Laboratory Immunology. 2001 ; Vol. 8, No. 1. pp. 170-173.
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Comparison of specific serological assays for diagnosing human herpesvirus 6 infection after liver transplantation. / Yoshikawa, T.; Black, J. B.; Ihira, M.; Suzuki, K.; Suga, S.; Iida, K.; Saito, Y.; Asonuma, K.; Tanaka, K.; Asano, Y.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 8, No. 1, 31.01.2001, p. 170-173.

Research output: Contribution to journalArticle

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T1 - Comparison of specific serological assays for diagnosing human herpesvirus 6 infection after liver transplantation

AU - Yoshikawa, T.

AU - Black, J. B.

AU - Ihira, M.

AU - Suzuki, K.

AU - Suga, S.

AU - Iida, K.

AU - Saito, Y.

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AU - Tanaka, K.

AU - Asano, Y.

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