Comparison of the tube test and column agglutination techniques for anti-A/-B antibody titration in healthy individuals

Hideaki Matsuura, Yoshiki Akatsuka, Takahiro Matsuno, Yukari Sugiura, Shoko Arakawa, Shota Oikawa, Jumpei Yoshida, Mitsuaki Kosugi, Nobuhiko Emi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background and Objectives: Determination of the anti-A/-B titre pre- and post-transplantation is beneficial for treatment selection. Currently, the recommended method for antibody titration is the tube test (TT) assay. Dithiothreitol (DTT) is used for IgM antibody inactivation. Recently, a fully automated antibody titration assay using the column agglutination technique (CAT) was developed (auto-CAT). Our aim was to compare the auto-CAT and TT techniques for ABO antibody titration, to evaluate the effectiveness of DTT-treated plasma for use with auto-CAT and to define the cut-off value for antibody titration by auto-CAT. Materials and Methods: We enrolled 30 healthy individuals, including 10 each for blood types A, B and O. We performed antibody titre measurement using the TT technique and auto-CAT simultaneously. Auto-CAT uses the bead column agglutination technology. Results: With the auto-CAT cut-off value set to weak (w)+ with DTT treatment plasma, the concordance rate was 45%, and the weighted kappa value between TT and auto-CAT results was 0·994 in all subjects. Furthermore, there was a significant positive correlation between the anti-A/-B titre results obtained using the TT technique and auto-CAT in all blood types. Moreover, a positive bias (falsely elevated end-points due to agglomeration of A/B cells) was not observed in auto-CAT testing using DTT-treated plasma. Conclusion: Our results show that 1+ agglutination using the TT technique is equivalent to w+ agglutination obtained using auto-CAT. We recommend that DTT may be used with auto-CAT to measure antibody titres. Thus, we suggest that auto-CAT is useful for antibody titration in routine examination.

Original languageEnglish
Pages (from-to)787-794
Number of pages8
JournalVox Sanguinis
Volume113
Issue number8
DOIs
Publication statusPublished - 01-11-2018

Fingerprint

Agglutination Tests
Agglutination
Antibodies
Dithiothreitol
Transplantation

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Matsuura, H., Akatsuka, Y., Matsuno, T., Sugiura, Y., Arakawa, S., Oikawa, S., ... Emi, N. (2018). Comparison of the tube test and column agglutination techniques for anti-A/-B antibody titration in healthy individuals. Vox Sanguinis, 113(8), 787-794. https://doi.org/10.1111/vox.12713
Matsuura, Hideaki ; Akatsuka, Yoshiki ; Matsuno, Takahiro ; Sugiura, Yukari ; Arakawa, Shoko ; Oikawa, Shota ; Yoshida, Jumpei ; Kosugi, Mitsuaki ; Emi, Nobuhiko. / Comparison of the tube test and column agglutination techniques for anti-A/-B antibody titration in healthy individuals. In: Vox Sanguinis. 2018 ; Vol. 113, No. 8. pp. 787-794.
@article{211f95e92a7b49c2ad42e813b660dc4c,
title = "Comparison of the tube test and column agglutination techniques for anti-A/-B antibody titration in healthy individuals",
abstract = "Background and Objectives: Determination of the anti-A/-B titre pre- and post-transplantation is beneficial for treatment selection. Currently, the recommended method for antibody titration is the tube test (TT) assay. Dithiothreitol (DTT) is used for IgM antibody inactivation. Recently, a fully automated antibody titration assay using the column agglutination technique (CAT) was developed (auto-CAT). Our aim was to compare the auto-CAT and TT techniques for ABO antibody titration, to evaluate the effectiveness of DTT-treated plasma for use with auto-CAT and to define the cut-off value for antibody titration by auto-CAT. Materials and Methods: We enrolled 30 healthy individuals, including 10 each for blood types A, B and O. We performed antibody titre measurement using the TT technique and auto-CAT simultaneously. Auto-CAT uses the bead column agglutination technology. Results: With the auto-CAT cut-off value set to weak (w)+ with DTT treatment plasma, the concordance rate was 45{\%}, and the weighted kappa value between TT and auto-CAT results was 0·994 in all subjects. Furthermore, there was a significant positive correlation between the anti-A/-B titre results obtained using the TT technique and auto-CAT in all blood types. Moreover, a positive bias (falsely elevated end-points due to agglomeration of A/B cells) was not observed in auto-CAT testing using DTT-treated plasma. Conclusion: Our results show that 1+ agglutination using the TT technique is equivalent to w+ agglutination obtained using auto-CAT. We recommend that DTT may be used with auto-CAT to measure antibody titres. Thus, we suggest that auto-CAT is useful for antibody titration in routine examination.",
author = "Hideaki Matsuura and Yoshiki Akatsuka and Takahiro Matsuno and Yukari Sugiura and Shoko Arakawa and Shota Oikawa and Jumpei Yoshida and Mitsuaki Kosugi and Nobuhiko Emi",
year = "2018",
month = "11",
day = "1",
doi = "10.1111/vox.12713",
language = "English",
volume = "113",
pages = "787--794",
journal = "Vox Sanguinis",
issn = "0042-9007",
publisher = "Wiley-Blackwell",
number = "8",

}

Matsuura, H, Akatsuka, Y, Matsuno, T, Sugiura, Y, Arakawa, S, Oikawa, S, Yoshida, J, Kosugi, M & Emi, N 2018, 'Comparison of the tube test and column agglutination techniques for anti-A/-B antibody titration in healthy individuals', Vox Sanguinis, vol. 113, no. 8, pp. 787-794. https://doi.org/10.1111/vox.12713

Comparison of the tube test and column agglutination techniques for anti-A/-B antibody titration in healthy individuals. / Matsuura, Hideaki; Akatsuka, Yoshiki; Matsuno, Takahiro; Sugiura, Yukari; Arakawa, Shoko; Oikawa, Shota; Yoshida, Jumpei; Kosugi, Mitsuaki; Emi, Nobuhiko.

In: Vox Sanguinis, Vol. 113, No. 8, 01.11.2018, p. 787-794.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Comparison of the tube test and column agglutination techniques for anti-A/-B antibody titration in healthy individuals

AU - Matsuura, Hideaki

AU - Akatsuka, Yoshiki

AU - Matsuno, Takahiro

AU - Sugiura, Yukari

AU - Arakawa, Shoko

AU - Oikawa, Shota

AU - Yoshida, Jumpei

AU - Kosugi, Mitsuaki

AU - Emi, Nobuhiko

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Background and Objectives: Determination of the anti-A/-B titre pre- and post-transplantation is beneficial for treatment selection. Currently, the recommended method for antibody titration is the tube test (TT) assay. Dithiothreitol (DTT) is used for IgM antibody inactivation. Recently, a fully automated antibody titration assay using the column agglutination technique (CAT) was developed (auto-CAT). Our aim was to compare the auto-CAT and TT techniques for ABO antibody titration, to evaluate the effectiveness of DTT-treated plasma for use with auto-CAT and to define the cut-off value for antibody titration by auto-CAT. Materials and Methods: We enrolled 30 healthy individuals, including 10 each for blood types A, B and O. We performed antibody titre measurement using the TT technique and auto-CAT simultaneously. Auto-CAT uses the bead column agglutination technology. Results: With the auto-CAT cut-off value set to weak (w)+ with DTT treatment plasma, the concordance rate was 45%, and the weighted kappa value between TT and auto-CAT results was 0·994 in all subjects. Furthermore, there was a significant positive correlation between the anti-A/-B titre results obtained using the TT technique and auto-CAT in all blood types. Moreover, a positive bias (falsely elevated end-points due to agglomeration of A/B cells) was not observed in auto-CAT testing using DTT-treated plasma. Conclusion: Our results show that 1+ agglutination using the TT technique is equivalent to w+ agglutination obtained using auto-CAT. We recommend that DTT may be used with auto-CAT to measure antibody titres. Thus, we suggest that auto-CAT is useful for antibody titration in routine examination.

AB - Background and Objectives: Determination of the anti-A/-B titre pre- and post-transplantation is beneficial for treatment selection. Currently, the recommended method for antibody titration is the tube test (TT) assay. Dithiothreitol (DTT) is used for IgM antibody inactivation. Recently, a fully automated antibody titration assay using the column agglutination technique (CAT) was developed (auto-CAT). Our aim was to compare the auto-CAT and TT techniques for ABO antibody titration, to evaluate the effectiveness of DTT-treated plasma for use with auto-CAT and to define the cut-off value for antibody titration by auto-CAT. Materials and Methods: We enrolled 30 healthy individuals, including 10 each for blood types A, B and O. We performed antibody titre measurement using the TT technique and auto-CAT simultaneously. Auto-CAT uses the bead column agglutination technology. Results: With the auto-CAT cut-off value set to weak (w)+ with DTT treatment plasma, the concordance rate was 45%, and the weighted kappa value between TT and auto-CAT results was 0·994 in all subjects. Furthermore, there was a significant positive correlation between the anti-A/-B titre results obtained using the TT technique and auto-CAT in all blood types. Moreover, a positive bias (falsely elevated end-points due to agglomeration of A/B cells) was not observed in auto-CAT testing using DTT-treated plasma. Conclusion: Our results show that 1+ agglutination using the TT technique is equivalent to w+ agglutination obtained using auto-CAT. We recommend that DTT may be used with auto-CAT to measure antibody titres. Thus, we suggest that auto-CAT is useful for antibody titration in routine examination.

UR - http://www.scopus.com/inward/record.url?scp=85053735971&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85053735971&partnerID=8YFLogxK

U2 - 10.1111/vox.12713

DO - 10.1111/vox.12713

M3 - Article

VL - 113

SP - 787

EP - 794

JO - Vox Sanguinis

JF - Vox Sanguinis

SN - 0042-9007

IS - 8

ER -