Competition between a noncoding exon and introns

Gomafu contains tandem UACUAAC repeats and associates with splicing factor-1

Hitomi Tsuiji, Rei Yoshimoto, Yuko Hasegawa, Masaaki Furuno, Minoru Yoshida, Shinichi Nakagawa

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Gomafu (also referred to as RNCR2/MIAT) was originally identified as a noncoding RNA expressed in a particular set of neurons. Unlike protein-coding mRNAs, the Gomafu RNA escapes nuclear export and stably accumulates in the nucleus, making a unique nuclear compartment. Although recent studies have revealed the functional relevance of Gomafu in a series of physiological processes, the underlying molecular mechanism remains largely uncharacterized. In this report, we identified a chicken homologue of Gomafu using a comparative genomic approach to search for functionally important and conserved sequence motifs among evolutionarily distant species. Unexpectedly, we found that all Gomafu RNA examined shared a distinctive feature: tandem repeats of UACUAAC, a sequence that has been identified as a conserved intron branch point in the yeast Saccharomyces cerevisiae. The tandem UACUAAC Gomafu RNA repeats bind to the SF1 splicing factor with a higher affinity than the divergent branch point sequence in mammals, which affects the kinetics of the splicing reaction in vitro. We propose that the Gomafu RNA regulates splicing efficiency by changing the local concentration of splicing factors within the nucleus.

Original languageEnglish
Pages (from-to)479-490
Number of pages12
JournalGenes to Cells
Volume16
Issue number5
DOIs
Publication statusPublished - 01-05-2011

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Tandem Repeat Sequences
Introns
Exons
RNA
Physiological Phenomena
RNA Splicing
Untranslated RNA
Cell Nucleus Active Transport
Conserved Sequence
Saccharomyces cerevisiae
Mammals
Chickens
Yeasts
Neurons
Messenger RNA
RNA Splicing Factors
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Tsuiji, Hitomi ; Yoshimoto, Rei ; Hasegawa, Yuko ; Furuno, Masaaki ; Yoshida, Minoru ; Nakagawa, Shinichi. / Competition between a noncoding exon and introns : Gomafu contains tandem UACUAAC repeats and associates with splicing factor-1. In: Genes to Cells. 2011 ; Vol. 16, No. 5. pp. 479-490.
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Competition between a noncoding exon and introns : Gomafu contains tandem UACUAAC repeats and associates with splicing factor-1. / Tsuiji, Hitomi; Yoshimoto, Rei; Hasegawa, Yuko; Furuno, Masaaki; Yoshida, Minoru; Nakagawa, Shinichi.

In: Genes to Cells, Vol. 16, No. 5, 01.05.2011, p. 479-490.

Research output: Contribution to journalArticle

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