Complete DNA sequence of the mitochondrial genome of the ascidian Halocynthia roretzi (Chordata, Urochordata)

Shin Ichi Yokobori, Takuya Ueda, Gertraud Feldmaier-Fuchs, Svante Pääbo, Rei Ueshima, Akiko Kondo, Kazuya Nishikawa, Kimitsuna Watanabe

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The complete nucleotide sequence of the 14,771-bp-long mitochondrial (mt) DNA of a urochordate (Chordata)-the ascidian Halocynthia roretzi-was determined. All the Halocynthia mt-genes were found to be located on a single strand, which is rich in T and G rather than in A and C. Like nematode and Mytilus edulis mtDNAs, that of Halocynthia encodes no ATP synthetase subunit 8 gene. However, it does encode an additional tRNA gene for glycine (anticodon TCT) that enables Halocynthia mitochondria to use AGA and AGG codons for glycine. The mtDNA carries an unusual tRNA(Met) gene with a TAT anticodon instead of the usual tRNA(Met)/(CAT) gene. As in other metazoan mtDNAs, there is not any long noncoding region. The gene order of Halocynthia mtDNA is completely different from that of vertebrate mtDNAs except for tRNA(His)-tRNA(Ser)/(GGU), suggesting that evolutionary change in the mt-gene structure is much accelerated in the urochordate line compared with that in vertebrates. The amino acid sequences of Halocynthia mtproteins deduced from their gene sequences are quite different from those in other metazoans, indicating that the substitution rate in Halocynthia mt-protein genes is also accelerated.

Original languageEnglish
Pages (from-to)1851-1862
Number of pages12
JournalGenetics
Volume153
Issue number4
Publication statusPublished - 01-12-1999

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Chordata
Urochordata
Mitochondrial Genome
Mitochondrial Genes
Mitochondrial DNA
RNA, Transfer, Met
Anticodon
Genes
Glycine
Vertebrates
RNA, Transfer, His
RNA, Transfer, Ser
Mytilus edulis
Gene Order
Mitochondrial Proteins
Ligases
Transfer RNA
Codon
Amino Acid Sequence
Mitochondria

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Yokobori, S. I., Ueda, T., Feldmaier-Fuchs, G., Pääbo, S., Ueshima, R., Kondo, A., ... Watanabe, K. (1999). Complete DNA sequence of the mitochondrial genome of the ascidian Halocynthia roretzi (Chordata, Urochordata). Genetics, 153(4), 1851-1862.
Yokobori, Shin Ichi ; Ueda, Takuya ; Feldmaier-Fuchs, Gertraud ; Pääbo, Svante ; Ueshima, Rei ; Kondo, Akiko ; Nishikawa, Kazuya ; Watanabe, Kimitsuna. / Complete DNA sequence of the mitochondrial genome of the ascidian Halocynthia roretzi (Chordata, Urochordata). In: Genetics. 1999 ; Vol. 153, No. 4. pp. 1851-1862.
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Yokobori, SI, Ueda, T, Feldmaier-Fuchs, G, Pääbo, S, Ueshima, R, Kondo, A, Nishikawa, K & Watanabe, K 1999, 'Complete DNA sequence of the mitochondrial genome of the ascidian Halocynthia roretzi (Chordata, Urochordata)', Genetics, vol. 153, no. 4, pp. 1851-1862.

Complete DNA sequence of the mitochondrial genome of the ascidian Halocynthia roretzi (Chordata, Urochordata). / Yokobori, Shin Ichi; Ueda, Takuya; Feldmaier-Fuchs, Gertraud; Pääbo, Svante; Ueshima, Rei; Kondo, Akiko; Nishikawa, Kazuya; Watanabe, Kimitsuna.

In: Genetics, Vol. 153, No. 4, 01.12.1999, p. 1851-1862.

Research output: Contribution to journalArticle

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AU - Yokobori, Shin Ichi

AU - Ueda, Takuya

AU - Feldmaier-Fuchs, Gertraud

AU - Pääbo, Svante

AU - Ueshima, Rei

AU - Kondo, Akiko

AU - Nishikawa, Kazuya

AU - Watanabe, Kimitsuna

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N2 - The complete nucleotide sequence of the 14,771-bp-long mitochondrial (mt) DNA of a urochordate (Chordata)-the ascidian Halocynthia roretzi-was determined. All the Halocynthia mt-genes were found to be located on a single strand, which is rich in T and G rather than in A and C. Like nematode and Mytilus edulis mtDNAs, that of Halocynthia encodes no ATP synthetase subunit 8 gene. However, it does encode an additional tRNA gene for glycine (anticodon TCT) that enables Halocynthia mitochondria to use AGA and AGG codons for glycine. The mtDNA carries an unusual tRNA(Met) gene with a TAT anticodon instead of the usual tRNA(Met)/(CAT) gene. As in other metazoan mtDNAs, there is not any long noncoding region. The gene order of Halocynthia mtDNA is completely different from that of vertebrate mtDNAs except for tRNA(His)-tRNA(Ser)/(GGU), suggesting that evolutionary change in the mt-gene structure is much accelerated in the urochordate line compared with that in vertebrates. The amino acid sequences of Halocynthia mtproteins deduced from their gene sequences are quite different from those in other metazoans, indicating that the substitution rate in Halocynthia mt-protein genes is also accelerated.

AB - The complete nucleotide sequence of the 14,771-bp-long mitochondrial (mt) DNA of a urochordate (Chordata)-the ascidian Halocynthia roretzi-was determined. All the Halocynthia mt-genes were found to be located on a single strand, which is rich in T and G rather than in A and C. Like nematode and Mytilus edulis mtDNAs, that of Halocynthia encodes no ATP synthetase subunit 8 gene. However, it does encode an additional tRNA gene for glycine (anticodon TCT) that enables Halocynthia mitochondria to use AGA and AGG codons for glycine. The mtDNA carries an unusual tRNA(Met) gene with a TAT anticodon instead of the usual tRNA(Met)/(CAT) gene. As in other metazoan mtDNAs, there is not any long noncoding region. The gene order of Halocynthia mtDNA is completely different from that of vertebrate mtDNAs except for tRNA(His)-tRNA(Ser)/(GGU), suggesting that evolutionary change in the mt-gene structure is much accelerated in the urochordate line compared with that in vertebrates. The amino acid sequences of Halocynthia mtproteins deduced from their gene sequences are quite different from those in other metazoans, indicating that the substitution rate in Halocynthia mt-protein genes is also accelerated.

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Yokobori SI, Ueda T, Feldmaier-Fuchs G, Pääbo S, Ueshima R, Kondo A et al. Complete DNA sequence of the mitochondrial genome of the ascidian Halocynthia roretzi (Chordata, Urochordata). Genetics. 1999 Dec 1;153(4):1851-1862.