TY - JOUR
T1 - Complete sequences of multidrug resistance plasmids bearing rmtD1 and rmtD2 16S rRNA methyltransferase genes
AU - Bueno, Maria Fernanda C.
AU - Francisco, Gabriela R.
AU - De Oliveira Garcia, Doroti
AU - Doi, Yohei
N1 - Funding Information:
M.F.C.B. and G.R.F. were supported through doctoral scholarships from FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo; grants 2014/23715-8 and 2015/10551). FAPESP provided funding to D.D.O.G. under research grant 2012/21709-5. The National Institutes of Health provided funding to Y.D. under research grants R01AI104895 and R21AI107302. Y.D. has served on advisory boards for Shionogi, Meiji Seika Pharma, and Tetraphase Pharmaceuticals, has consulted for Melinta Therapeutics, and has received research funding from Merck for studies unrelated to this work. All other authors declare no conflicts of interest. FAPESP provided funding (doctoral scholarship) to Maria Fernanda Campagnari Bueno and Gabriela Rodrigues Francisco under grant numbers 2014/23715-8 and 2015/10551. FAPESP provided funding to Doroti de Oliveira Garcia under grant number 2012/21709-5. Office of Extramural Research, National Institutes of Health (OER) provided funding to Yohei Doi under grant number R01AI104895. Office of Extramural Research, National Institutes of Health (OER) provided funding to Yohei Doi under grant number R21AI107302.
Publisher Copyright:
Copyright © 2016, American Society for Microbiology. All Rights Reserved.
PY - 2016/3
Y1 - 2016/3
N2 - Complete nucleotide sequences were determined for two plasmids bearing rmtD group 16S rRNA methyltransferase genes. pKp64/11 was 78 kb in size, belonged to the IncL/M group, and harbored blaTEM-1b, sul1, qacEΔ1, dfrA22, and rmtD1 across two multidrug resistance regions (MRRs). pKp368/10 was 170 kb in size, belonged to the IncA/C group, and harbored acrB, sul1, qacEΔ1, ant(3")-Ia, aac(6')-Ib, cat, rmtD2, and blaCTXM-8 across three MRRs. The rmtD-containing regions shared a conserved motif, suggesting a common origin for the two rmtD alleles.
AB - Complete nucleotide sequences were determined for two plasmids bearing rmtD group 16S rRNA methyltransferase genes. pKp64/11 was 78 kb in size, belonged to the IncL/M group, and harbored blaTEM-1b, sul1, qacEΔ1, dfrA22, and rmtD1 across two multidrug resistance regions (MRRs). pKp368/10 was 170 kb in size, belonged to the IncA/C group, and harbored acrB, sul1, qacEΔ1, ant(3")-Ia, aac(6')-Ib, cat, rmtD2, and blaCTXM-8 across three MRRs. The rmtD-containing regions shared a conserved motif, suggesting a common origin for the two rmtD alleles.
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U2 - 10.1128/AAC.02562-15
DO - 10.1128/AAC.02562-15
M3 - Article
C2 - 26729503
AN - SCOPUS:84960145316
VL - 60
SP - 1928
EP - 1931
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
SN - 0066-4804
IS - 3
ER -