Component of splicing factor SF3b plays a key role in translational control of polyribosomes on the endoplasmic reticulum

Tomonori Ueno, Yuki Taga, Rei Yoshimoto, Akira Maeda, Shunji Hattori, Kiyoko Ogawa-Goto

Research output: Contribution to journalArticle

Abstract

One of the morphological hallmarks of terminally differentiated secretory cells is highly proliferated membrane of the rough endoplasmic reticulum (ER), but the molecular basis for the high rate of protein biosynthesis in these cells remains poorly documented. An important aspect of ER translational control is the molecular mechanism that supports efficient use of targeted mRNAs in polyribosomes. Here, we identify an enhancement system for ER translation promoted by p180, an integral ER membrane protein we previously reported as an essential factor for the assembly of ER polyribosomes. We provide evidence that association of target mRNAs with p180 is critical for efficient translation, and that SF3b4, an RNA-binding protein in the splicing factor SF3b, functions as a cofactor for p180 at the ER and plays a key role in enhanced translation of secretory proteins. A cis-element in the 5′ untranslated region of collagen and fibronectin genes is important to increase translational efficiency in the presence of p180 and SF3b4. These data demonstrate that a unique system comprising a p180–SF3b4–mRNA complex facilitates the selective assembly of polyribosomes on the ER.

Original languageEnglish
Pages (from-to)9340-9349
Number of pages10
JournalProceedings of the National Academy of Sciences of the United States of America
Volume116
Issue number19
DOIs
Publication statusPublished - 07-05-2019

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Polyribosomes
Endoplasmic Reticulum
Protein Biosynthesis
Protein Splicing
Messenger RNA
RNA-Binding Proteins
Rough Endoplasmic Reticulum
5' Untranslated Regions
Fibronectins
RNA Splicing Factors
Membrane Proteins
Collagen
Membranes
Genes

All Science Journal Classification (ASJC) codes

  • General

Cite this

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title = "Component of splicing factor SF3b plays a key role in translational control of polyribosomes on the endoplasmic reticulum",
abstract = "One of the morphological hallmarks of terminally differentiated secretory cells is highly proliferated membrane of the rough endoplasmic reticulum (ER), but the molecular basis for the high rate of protein biosynthesis in these cells remains poorly documented. An important aspect of ER translational control is the molecular mechanism that supports efficient use of targeted mRNAs in polyribosomes. Here, we identify an enhancement system for ER translation promoted by p180, an integral ER membrane protein we previously reported as an essential factor for the assembly of ER polyribosomes. We provide evidence that association of target mRNAs with p180 is critical for efficient translation, and that SF3b4, an RNA-binding protein in the splicing factor SF3b, functions as a cofactor for p180 at the ER and plays a key role in enhanced translation of secretory proteins. A cis-element in the 5′ untranslated region of collagen and fibronectin genes is important to increase translational efficiency in the presence of p180 and SF3b4. These data demonstrate that a unique system comprising a p180–SF3b4–mRNA complex facilitates the selective assembly of polyribosomes on the ER.",
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Component of splicing factor SF3b plays a key role in translational control of polyribosomes on the endoplasmic reticulum. / Ueno, Tomonori; Taga, Yuki; Yoshimoto, Rei; Maeda, Akira; Hattori, Shunji; Ogawa-Goto, Kiyoko.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 116, No. 19, 07.05.2019, p. 9340-9349.

Research output: Contribution to journalArticle

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