Compound 48/80, a mast cell degranulator, causes oxidative damage by enhancing Vitamin C synthesis via reduced glutathione depletion and lipid peroxidation through neutrophil infiltration in rat livers

Yosihiji Ohta, Koji Yashiro, Koji Ohashi, Yosuke Horikoshi, Chiaki Kusumoto, Tatsuya Matsura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In this study, we examined whether compound 48/80 (C48/80), a mast cell degranulator, causes hepatic oxidative damage in rats. Serum and liver biochemical parameters were determined 0.5, 3 or 6 h after a single treatment with C48/80 (0.75 mg/kg). Serum histamine and serotonin levels increased 0.5 h after C48/80 treatment but diminished thereafter. Increases in serum Vitamin C (VC) and transaminases and hepatic hydrogen peroxide, lipid peroxide, and myeloperoxidase levels and a decrease in hepatic reduced glutathione level occurred 0.5 h after C48/80 treatment and further proceeded at 3 h, but these changes diminished at 6 h. Serum lipid peroxide and hepatic VC levels increased 3 h after C48/80 treatment. Hepatic glycogen level decreased 0.5 h after C48/80 treatment and further decreased at 3 h. Pre-administered ketotifen diminished all these changes found at 3 h after treatment, while pre-administered NPC 14686 diminished these changes except changes in serum histamine and serotonin levels. Hepatocellular apoptosis observed at 3 h after C48/80 treatment was attenuated by pre-administered ketotifen and NPC 14686. These results indicate that C48/80 causes oxidative damage by enhancing VC synthesis via reduced glutathione depletion-dependent glycogenosis and lipid peroxidation through neutrophil infiltration following mast cell degranulation in rat livers.

Original languageEnglish
Pages (from-to)187-198
Number of pages12
JournalJournal of Clinical Biochemistry and Nutrition
Volume60
Issue number3
DOIs
Publication statusPublished - 01-05-2017

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p-Methoxy-N-methylphenethylamine
Neutrophil Infiltration
Infiltration
Mast Cells
Liver
Lipid Peroxidation
Ascorbic Acid
Glutathione
Rats
Lipids
Ketotifen
Serum
Lipid Peroxides
Histamine
Serotonin
Glycogen Storage Disease
Cell Degranulation
Liver Glycogen
Transaminases
Hydrogen Peroxide

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

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abstract = "In this study, we examined whether compound 48/80 (C48/80), a mast cell degranulator, causes hepatic oxidative damage in rats. Serum and liver biochemical parameters were determined 0.5, 3 or 6 h after a single treatment with C48/80 (0.75 mg/kg). Serum histamine and serotonin levels increased 0.5 h after C48/80 treatment but diminished thereafter. Increases in serum Vitamin C (VC) and transaminases and hepatic hydrogen peroxide, lipid peroxide, and myeloperoxidase levels and a decrease in hepatic reduced glutathione level occurred 0.5 h after C48/80 treatment and further proceeded at 3 h, but these changes diminished at 6 h. Serum lipid peroxide and hepatic VC levels increased 3 h after C48/80 treatment. Hepatic glycogen level decreased 0.5 h after C48/80 treatment and further decreased at 3 h. Pre-administered ketotifen diminished all these changes found at 3 h after treatment, while pre-administered NPC 14686 diminished these changes except changes in serum histamine and serotonin levels. Hepatocellular apoptosis observed at 3 h after C48/80 treatment was attenuated by pre-administered ketotifen and NPC 14686. These results indicate that C48/80 causes oxidative damage by enhancing VC synthesis via reduced glutathione depletion-dependent glycogenosis and lipid peroxidation through neutrophil infiltration following mast cell degranulation in rat livers.",
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Compound 48/80, a mast cell degranulator, causes oxidative damage by enhancing Vitamin C synthesis via reduced glutathione depletion and lipid peroxidation through neutrophil infiltration in rat livers. / Ohta, Yosihiji; Yashiro, Koji; Ohashi, Koji; Horikoshi, Yosuke; Kusumoto, Chiaki; Matsura, Tatsuya.

In: Journal of Clinical Biochemistry and Nutrition, Vol. 60, No. 3, 01.05.2017, p. 187-198.

Research output: Contribution to journalArticle

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T1 - Compound 48/80, a mast cell degranulator, causes oxidative damage by enhancing Vitamin C synthesis via reduced glutathione depletion and lipid peroxidation through neutrophil infiltration in rat livers

AU - Ohta, Yosihiji

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AU - Ohashi, Koji

AU - Horikoshi, Yosuke

AU - Kusumoto, Chiaki

AU - Matsura, Tatsuya

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