Compound 48/80 causes oxidative stress in the adrenal gland of rats through mast cell degranulation

Shingo Kaida, Yoshiji Ohta, Yoichiro Imai, Koji Ohashi, Minoru Kawanishi

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Rats were intraperitoneally treated once with compound 48/80 (C48/80), a mast cell degranulator, (0.75 mg/kg). Serum serotonin, histamine and corticosterone levels increased 0.5 h after C48/80 treatment, but their increases were reduced thereafter. Adrenal total ascorbic acid (ascorbic acid plus dehydroascorbic acid), ascorbic acid and dehydroascorbic acid levels decreased 0.5, 3 or 6 h after C48/80 treatment, adrenal lipid peroxide level increased at 3 and 6 h, adrenal non-protein-SH level decreased at 3 and 6 h and adrenal β-tocopherol level decreased at 3 h. Ketotifen, a mast cell stabilizer (1 mg/kg) administered intraperitoneally at 0.5 h before C48/80 treatment, attenuated all these changes found in the serum and adrenal at 3 h after treatment, while β-tocopherol (250 mg/kg), administered orally at 0.5 h after C48/80 treatment, attenuated all these changes in the adrenal tissue. These results indicate that C48/80 causes oxidative stress in rat adrenal gland through mast cell degranulation.

Original languageEnglish
Pages (from-to)171-180
Number of pages10
JournalFree Radical Research
Issue number2
Publication statusPublished - 25-01-2010


All Science Journal Classification (ASJC) codes

  • Biochemistry

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