Compound 48/80 causes oxidative stress in the adrenal gland of rats through mast cell degranulation

Shingo Kaida, Yoshiji Ohta, Yoichiro Imai, Koji Ohashi, Minoru Kawanishi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Rats were intraperitoneally treated once with compound 48/80 (C48/80), a mast cell degranulator, (0.75 mg/kg). Serum serotonin, histamine and corticosterone levels increased 0.5 h after C48/80 treatment, but their increases were reduced thereafter. Adrenal total ascorbic acid (ascorbic acid plus dehydroascorbic acid), ascorbic acid and dehydroascorbic acid levels decreased 0.5, 3 or 6 h after C48/80 treatment, adrenal lipid peroxide level increased at 3 and 6 h, adrenal non-protein-SH level decreased at 3 and 6 h and adrenal β-tocopherol level decreased at 3 h. Ketotifen, a mast cell stabilizer (1 mg/kg) administered intraperitoneally at 0.5 h before C48/80 treatment, attenuated all these changes found in the serum and adrenal at 3 h after treatment, while β-tocopherol (250 mg/kg), administered orally at 0.5 h after C48/80 treatment, attenuated all these changes in the adrenal tissue. These results indicate that C48/80 causes oxidative stress in rat adrenal gland through mast cell degranulation.

Original languageEnglish
Pages (from-to)171-180
Number of pages10
JournalFree Radical Research
Volume44
Issue number2
DOIs
Publication statusPublished - 25-01-2010

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p-Methoxy-N-methylphenethylamine
Cell Degranulation
Oxidative stress
Adrenal Glands
Mast Cells
Rats
Oxidative Stress
Dehydroascorbic Acid
Ascorbic Acid
Tocopherols
Ketotifen
Lipid Peroxides
Corticosterone
Serum
Histamine
Serotonin
Tissue

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Kaida, Shingo ; Ohta, Yoshiji ; Imai, Yoichiro ; Ohashi, Koji ; Kawanishi, Minoru. / Compound 48/80 causes oxidative stress in the adrenal gland of rats through mast cell degranulation. In: Free Radical Research. 2010 ; Vol. 44, No. 2. pp. 171-180.
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Compound 48/80 causes oxidative stress in the adrenal gland of rats through mast cell degranulation. / Kaida, Shingo; Ohta, Yoshiji; Imai, Yoichiro; Ohashi, Koji; Kawanishi, Minoru.

In: Free Radical Research, Vol. 44, No. 2, 25.01.2010, p. 171-180.

Research output: Contribution to journalArticle

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