Comprehensive behavioral analysis of calcium/calmodulin-dependent protein kinase IV knockout mice

Keizo Takao, Koichi Tanda, Kenji Nakamura, Jiro Kasahara, Kazuki Nakao, Motoya Katsuki, Kazuo Nakanishi, Nobuyuki Yamasaki, Keiko Toyama, Minami Adachi, Masahiro Umeda, Tsutomu Araki, Kohji Fukunaga, Hisatake Kondo, Hiroyuki Sakagami, Tsuyoshi Miyakawa

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Calcium-calmodulin dependent protein kinase IV (CaMKIV) is a protein kinase that activates the transcription factor CREB, the cyclic AMP-response element binding protein. CREB is a key transcription factor in synaptic plasticity and memory consolidation. To elucidate the behavioral effects of CaMKIV deficiency, we subjected CaMKIV knockout (CaMKIV KO) mice to a battery of behavioral tests. CaMKIV KO had no significant effects on locomotor activity, motor coordination, social interaction, pain sensitivity, prepulse inhibition, attention, or depression-like behavior. Consistent with previous reports, CaMKIV KO mice exhibited impaired retention in a fear conditioning test 28 days after training. In contrast, however, CaMKIV KO mice did not show any testing performance deficits in passive avoidance, one of the most commonly used fear memory paradigms, 28 days after training, suggesting that remote fear memory is intact. CaMKIV KO mice exhibited intact spatial reference memory learning in the Barnes circular maze, and normal spatial working memory in an eight-arm radial maze. CaMKIV KO mice also showed mildly decreased anxiety-like behavior, suggesting that CaMKIV is involved in regulating emotional behavior. These findings indicate that CaMKIV might not be essential for fear memory or spatial memory, although it is possible that the activities of other neural mechanisms or signaling pathways compensate for the CaMKIV deficiency.

Original languageEnglish
Article numbere9460
JournalPloS one
Volume5
Issue number3
DOIs
Publication statusPublished - 01-03-2010

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Calcium-Calmodulin-Dependent Protein Kinase Type 4
calmodulin
Knockout Mice
protein kinases
mice
Data storage equipment
fearfulness
Fear
calcium-calmodulin-dependent protein kinase
Transcription Factors
transcription factors
Cyclic AMP Response Element-Binding Protein
Neuronal Plasticity
Long-Term Memory
response elements
testing
conditioned behavior
cyclic AMP
Locomotion
anxiety

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Takao, Keizo ; Tanda, Koichi ; Nakamura, Kenji ; Kasahara, Jiro ; Nakao, Kazuki ; Katsuki, Motoya ; Nakanishi, Kazuo ; Yamasaki, Nobuyuki ; Toyama, Keiko ; Adachi, Minami ; Umeda, Masahiro ; Araki, Tsutomu ; Fukunaga, Kohji ; Kondo, Hisatake ; Sakagami, Hiroyuki ; Miyakawa, Tsuyoshi. / Comprehensive behavioral analysis of calcium/calmodulin-dependent protein kinase IV knockout mice. In: PloS one. 2010 ; Vol. 5, No. 3.
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abstract = "Calcium-calmodulin dependent protein kinase IV (CaMKIV) is a protein kinase that activates the transcription factor CREB, the cyclic AMP-response element binding protein. CREB is a key transcription factor in synaptic plasticity and memory consolidation. To elucidate the behavioral effects of CaMKIV deficiency, we subjected CaMKIV knockout (CaMKIV KO) mice to a battery of behavioral tests. CaMKIV KO had no significant effects on locomotor activity, motor coordination, social interaction, pain sensitivity, prepulse inhibition, attention, or depression-like behavior. Consistent with previous reports, CaMKIV KO mice exhibited impaired retention in a fear conditioning test 28 days after training. In contrast, however, CaMKIV KO mice did not show any testing performance deficits in passive avoidance, one of the most commonly used fear memory paradigms, 28 days after training, suggesting that remote fear memory is intact. CaMKIV KO mice exhibited intact spatial reference memory learning in the Barnes circular maze, and normal spatial working memory in an eight-arm radial maze. CaMKIV KO mice also showed mildly decreased anxiety-like behavior, suggesting that CaMKIV is involved in regulating emotional behavior. These findings indicate that CaMKIV might not be essential for fear memory or spatial memory, although it is possible that the activities of other neural mechanisms or signaling pathways compensate for the CaMKIV deficiency.",
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Takao, K, Tanda, K, Nakamura, K, Kasahara, J, Nakao, K, Katsuki, M, Nakanishi, K, Yamasaki, N, Toyama, K, Adachi, M, Umeda, M, Araki, T, Fukunaga, K, Kondo, H, Sakagami, H & Miyakawa, T 2010, 'Comprehensive behavioral analysis of calcium/calmodulin-dependent protein kinase IV knockout mice', PloS one, vol. 5, no. 3, e9460. https://doi.org/10.1371/journal.pone.0009460

Comprehensive behavioral analysis of calcium/calmodulin-dependent protein kinase IV knockout mice. / Takao, Keizo; Tanda, Koichi; Nakamura, Kenji; Kasahara, Jiro; Nakao, Kazuki; Katsuki, Motoya; Nakanishi, Kazuo; Yamasaki, Nobuyuki; Toyama, Keiko; Adachi, Minami; Umeda, Masahiro; Araki, Tsutomu; Fukunaga, Kohji; Kondo, Hisatake; Sakagami, Hiroyuki; Miyakawa, Tsuyoshi.

In: PloS one, Vol. 5, No. 3, e9460, 01.03.2010.

Research output: Contribution to journalArticle

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T1 - Comprehensive behavioral analysis of calcium/calmodulin-dependent protein kinase IV knockout mice

AU - Takao, Keizo

AU - Tanda, Koichi

AU - Nakamura, Kenji

AU - Kasahara, Jiro

AU - Nakao, Kazuki

AU - Katsuki, Motoya

AU - Nakanishi, Kazuo

AU - Yamasaki, Nobuyuki

AU - Toyama, Keiko

AU - Adachi, Minami

AU - Umeda, Masahiro

AU - Araki, Tsutomu

AU - Fukunaga, Kohji

AU - Kondo, Hisatake

AU - Sakagami, Hiroyuki

AU - Miyakawa, Tsuyoshi

PY - 2010/3/1

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N2 - Calcium-calmodulin dependent protein kinase IV (CaMKIV) is a protein kinase that activates the transcription factor CREB, the cyclic AMP-response element binding protein. CREB is a key transcription factor in synaptic plasticity and memory consolidation. To elucidate the behavioral effects of CaMKIV deficiency, we subjected CaMKIV knockout (CaMKIV KO) mice to a battery of behavioral tests. CaMKIV KO had no significant effects on locomotor activity, motor coordination, social interaction, pain sensitivity, prepulse inhibition, attention, or depression-like behavior. Consistent with previous reports, CaMKIV KO mice exhibited impaired retention in a fear conditioning test 28 days after training. In contrast, however, CaMKIV KO mice did not show any testing performance deficits in passive avoidance, one of the most commonly used fear memory paradigms, 28 days after training, suggesting that remote fear memory is intact. CaMKIV KO mice exhibited intact spatial reference memory learning in the Barnes circular maze, and normal spatial working memory in an eight-arm radial maze. CaMKIV KO mice also showed mildly decreased anxiety-like behavior, suggesting that CaMKIV is involved in regulating emotional behavior. These findings indicate that CaMKIV might not be essential for fear memory or spatial memory, although it is possible that the activities of other neural mechanisms or signaling pathways compensate for the CaMKIV deficiency.

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