Comprehensive behavioral analysis of cluster of differentiation 47 knockout mice

Hisatsugu Koshimizu, Keizo Takao, Takashi Matozaki, Hiroshi Ohnishi, Tsuyoshi Miyakawa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cluster of differentiation 47 (CD47) is a member of the immunoglobulin superfamily which functions as a ligand for the extracellular region of signal regulatory protein α (SIRPα), a protein which is abundantly expressed in the brain. Previous studies, including ours, have demonstrated that both CD47 and SIRPα fulfill various functions in the central nervous system (CNS), such as the modulation of synaptic transmission and neuronal cell survival. We previously reported that CD47 is involved in the regulation of depression-like behavior of mice in the forced swim test through its modulation of tyrosine phosphorylation of SIRPα. However, other potential behavioral functions of CD47 remain largely unknown. In this study, in an effort to further investigate functional roles of CD47 in the CNS, CD47 knockout (KO) mice and their wild-type littermates were subjected to a battery of behavioral tests. CD47 KO mice displayed decreased prepulse inhibition, while the startle response did not differ between genotypes. The mutants exhibited slightly but significantly decreased sociability and social novelty preference in Crawley's three-chamber social approach test, whereas in social interaction tests in which experimental and stimulus mice have direct contact with each other in a freely moving setting in a novel environment or home cage, there were no significant differences between the genotypes. While previous studies suggested that CD47 regulates fear memory in the inhibitory avoidance test in rodents, our CD47 KO mice exhibited normal fear and spatial memory in the fear conditioning and the Barnes maze tests, respectively. These findings suggest that CD47 is potentially involved in the regulation of sensorimotor gating and social behavior in mice.

Original languageEnglish
Article numbere89584
JournalPloS one
Volume9
Issue number2
DOIs
Publication statusPublished - 24-02-2014

Fingerprint

Knockout Mice
Cluster Analysis
cluster analysis
Fear
regulatory proteins
mice
Neurology
fearfulness
Startle Reflex
Proteins
Central Nervous System
Genotype
testing
Modulation
Sensory Gating
Data storage equipment
Phosphorylation
central nervous system
Social Behavior
Nucleic Acid Regulatory Sequences

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Koshimizu, Hisatsugu ; Takao, Keizo ; Matozaki, Takashi ; Ohnishi, Hiroshi ; Miyakawa, Tsuyoshi. / Comprehensive behavioral analysis of cluster of differentiation 47 knockout mice. In: PloS one. 2014 ; Vol. 9, No. 2.
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Comprehensive behavioral analysis of cluster of differentiation 47 knockout mice. / Koshimizu, Hisatsugu; Takao, Keizo; Matozaki, Takashi; Ohnishi, Hiroshi; Miyakawa, Tsuyoshi.

In: PloS one, Vol. 9, No. 2, e89584, 24.02.2014.

Research output: Contribution to journalArticle

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